Effect of AS-ODNs on p210BCR-ABLExpression
| Sample . | Control . | BreakpointSpecific AS-ODN . | Missense ODN . | AS-ODN to Other Breakpoint . |
|---|---|---|---|---|
| 1 | 1.0 | 0.43 | 0.76 | 1.52 |
| 2 | 1.0 | 0.27 | 1.42 | 0.86 |
| 3 | 1.0 | 0.44 | 0.85 | 1.94 |
| 4 | 1.0 | 0.17 | 0.53 | 0.64 |
| 5 | 1.0 | 0.75 | 0.9 | 0.84 |
| Mean ± SEM | 1.0 ± 0 | 0.41 ± 0.1 | 0.9 ± 0.15 | 1.16 ± 0.25 |
| Significance (compared with control) | P < .004 | NS | NS |
| Sample . | Control . | BreakpointSpecific AS-ODN . | Missense ODN . | AS-ODN to Other Breakpoint . |
|---|---|---|---|---|
| 1 | 1.0 | 0.43 | 0.76 | 1.52 |
| 2 | 1.0 | 0.27 | 1.42 | 0.86 |
| 3 | 1.0 | 0.44 | 0.85 | 1.94 |
| 4 | 1.0 | 0.17 | 0.53 | 0.64 |
| 5 | 1.0 | 0.75 | 0.9 | 0.84 |
| Mean ± SEM | 1.0 ± 0 | 0.41 ± 0.1 | 0.9 ± 0.15 | 1.16 ± 0.25 |
| Significance (compared with control) | P < .004 | NS | NS |
DR+ cells were incubated in serum-free medium for 96 hours with or without addition of breakpoint-specific and control ODNs. Protein extracts obtained from 1 to 2 × 10e6 cells were subjected to gel electrophoresis and Western blotting with an anti–c-ABL antibody as described in Materials and Methods. Densitometry was performed and results expressed as the ratio of p210BCR-ABL to p140c-ABL, with controls normalized to 1.0.
Abbreviation: NS, not significant.