Binding of Anti-CD44 MoAbs to Plastic-Bound CD44 Rgs
| CD44 Construct* . | MoAb-151 . | ||||
|---|---|---|---|---|---|
| 31A . | S5 . | IM7 . | S3 . | MEM-85 . | |
| CD44D-Rg | − | + | + | + | − |
| CD44H-Rg | − | + | + | + | + |
| F1-B Rg | − | − | − | − | + |
| F1-C Rg | − | − | − | − | + |
| F2 Rg | − | + | + | + | + |
| F3 Rg | − | + | + | + | + |
| CD44 Construct* . | MoAb-151 . | ||||
|---|---|---|---|---|---|
| 31A . | S5 . | IM7 . | S3 . | MEM-85 . | |
| CD44D-Rg | − | + | + | + | − |
| CD44H-Rg | − | + | + | + | + |
| F1-B Rg | − | − | − | − | + |
| F1-C Rg | − | − | − | − | + |
| F2 Rg | − | + | + | + | + |
| F3 Rg | − | + | + | + | + |
*CD44D-Rg encoded the full-length extracellular domain of canine CD44. CD44H-Rg encoded the full-length extracellular domain of human CD44. F1-B Rg encoded the initial 131 amino acids of CD44, which includes the hyaluronan binding domain. F1-C Rg, F2 Rg, and F3 Rg encoded successively larger proteins (amino terminal 145, 186, and 210 amino acids), with F1 and F2 encoding the region of CD44 that is highly conserved among species. The F3 Rg protein encoded a 24-amino acid region of nonconserved residues.
31A was a negative isotype control MoAb that recognized murine Thy-1 and did not cross-react with human or canine cells. IM7, S3, and MEM-85 were other anti-CD44 MoAbs. MEM-85 was a positive control that recognized both the mature human CD44 and all truncation variants, but did not cross-react with canine CD44.