Amino Acid Sequence Analysis of the 115-kD Band
Proteins Assigned . | Amino Acid Recovered at Each Cycle (pmoles of PTH-Amino Acid) . | ||||
---|---|---|---|---|---|
1 . | 2 . | 3 . | 4 . | 5 . | |
γ-chain | Y | V | A | T | R |
(3.5) | (3.8) | (4.2) | t* | (3.9) | |
α-chain | G | P | R | V | V |
(6.4) | (6.1) | (3.8) | (4.2) | (7.4) | |
Albumin | D | A | H | K | S |
(0.9) | (1.2) | (0.9) | (1.2) | t* | |
α2-PI | N | Q | E | Q | V |
(1.2) | nd | (1.5) | (1.2) | (7.4) |
Proteins Assigned . | Amino Acid Recovered at Each Cycle (pmoles of PTH-Amino Acid) . | ||||
---|---|---|---|---|---|
1 . | 2 . | 3 . | 4 . | 5 . | |
γ-chain | Y | V | A | T | R |
(3.5) | (3.8) | (4.2) | t* | (3.9) | |
α-chain | G | P | R | V | V |
(6.4) | (6.1) | (3.8) | (4.2) | (7.4) | |
Albumin | D | A | H | K | S |
(0.9) | (1.2) | (0.9) | (1.2) | t* | |
α2-PI | N | Q | E | Q | V |
(1.2) | nd | (1.5) | (1.2) | (7.4) |
*Trace amounts were detected.
Abbreviation: nd, not detected.