Clonogenic Features of CD34+CD44-6v+ and CD34+ CD44-6V−Cells
| . | CFU-M (%) . | CFU-G (%) . | CFU-GM (%) . | BFU-E (%) . | CFU-GEMM (%) . |
|---|---|---|---|---|---|
| CD34+ CD44-6v+ cells | 44* ± 7 | 19 ± 5 | 22 ± 5 | 10 ± 2 | 4 ± 1 |
| CD34+ CD44-6vneg cells | 24* ± 5 | 18 ± 7 | 20 ± 7 | 31 ± 8 | 7 ± 1 |
| . | CFU-M (%) . | CFU-G (%) . | CFU-GM (%) . | BFU-E (%) . | CFU-GEMM (%) . |
|---|---|---|---|---|---|
| CD34+ CD44-6v+ cells | 44* ± 7 | 19 ± 5 | 22 ± 5 | 10 ± 2 | 4 ± 1 |
| CD34+ CD44-6vneg cells | 24* ± 5 | 18 ± 7 | 20 ± 7 | 31 ± 8 | 7 ± 1 |
CD34+ CD44-6v+ and CD34+CD44-6vneg were sorted as detailed in Materials and Methods, and seeded at 2.103 cells/mL complete methyl cellulose medium supplemented with stimulating factors as described; CFU-M, CFU-GM, BFU-E, and CFU-GEMM were scored at day 16 (methyl cellulose assay). Results were expressed as the percentage of each type of colonies. All cultures were made in triplicate. Data are the means ± SE of three independent experiments.
The difference between data marked is statistically significant (Student's t-test, P < .05).