Comparisons of Gene Transfer Efficiencies Measured by Assessing G418-Resistance and PCR Detection of the NEO Gene in Individual Colonies for Human CFC Obtained From NOD/SCID Mice
| Method . | % Marked CFC . | |||
|---|---|---|---|---|
| Experiment 1 . | Experiment 2 . | Experiment 3 . | Mean ± SEM . | |
| G418-resistance | 10 | 11 | 7 | 9 ± 2 |
| (13, 18, 5, 3) | (10, 11) | (14, 7, 2, 4) | ||
| PCR | 43 | 11 | 41 | 32 ± 10 |
| (30, 48, 50, 44) | (0, 22) | (80, 30, 13, ND) | ||
| Method . | % Marked CFC . | |||
|---|---|---|---|---|
| Experiment 1 . | Experiment 2 . | Experiment 3 . | Mean ± SEM . | |
| G418-resistance | 10 | 11 | 7 | 9 ± 2 |
| (13, 18, 5, 3) | (10, 11) | (14, 7, 2, 4) | ||
| PCR | 43 | 11 | 41 | 32 ± 10 |
| (30, 48, 50, 44) | (0, 22) | (80, 30, 13, ND) | ||
Values shown for the individual experiments are means of the values shown in parentheses, which represent the proportion of marked CFC determined in individual mice. The mean number ± SEM of colonies counted in the absence of G418 was 28 ± 3 (range, 6 to 53) and 7 ± 1 (range, 1 to 16) in the presence of G418. For the PCR analyses, duplicate analyses of from 8 to 20 colonies were performed per mouse.
Abbreviation: ND, not done.