Output of Human Myeloid CFC and CD19+Cells From NOD/SCID Mice Engrafted With Cells Obtained From Cultures of CB Cells That Contained SFM for the First 3 Days and SFM or FCS Replete Medium for the Final 2 Days
| Experiment No. . | Culture Condition . | Time of Assessment of Engrafted Mice . | No. of Human Cells Regenerated in Each Mouse Tested . | |
|---|---|---|---|---|
| CFC (×103) . | CD19+ Cells (×106) . | |||
| 1 | FCS | 6 wks | 32, 25 | 2.3, 4.9 |
| SFM | 6 wks | 3, 8 | 2.1, 1.9 | |
| 2 | FCS | 8 wks | 2 | 0.8 |
| SFM | 8 wks | 2 | 2.0 | |
| 3 | FCS | 15 wks | 83, 7 | 37, 34 |
| SFM | 15 wks | 81, 150, 89 | 16, 59, 26 | |
| Experiment No. . | Culture Condition . | Time of Assessment of Engrafted Mice . | No. of Human Cells Regenerated in Each Mouse Tested . | |
|---|---|---|---|---|
| CFC (×103) . | CD19+ Cells (×106) . | |||
| 1 | FCS | 6 wks | 32, 25 | 2.3, 4.9 |
| SFM | 6 wks | 3, 8 | 2.1, 1.9 | |
| 2 | FCS | 8 wks | 2 | 0.8 |
| SFM | 8 wks | 2 | 2.0 | |
| 3 | FCS | 15 wks | 83, 7 | 37, 34 |
| SFM | 15 wks | 81, 150, 89 | 16, 59, 26 | |
Values shown are the results obtained from individual mice calculated in each case, assuming two femurs and two tibias contain 25% of the total marrow population. Each of the three experiments was initiated with lin− CB cells and the output values shown have been adjusted to correspond to the yield of progeny expected from 105 input CD34+ CB cells originally placed in culture.
Abbreviation: SFM, serum-free medium.