Adhesion and Proliferative Response of EML-C1 Cells to FN and Its Fragments
| Proteins . | % Adhesion* . | CFU-GM Production-151 . |
|---|---|---|
| BSA | 8.3 ± 2.6 | 67 ± 3 |
| FN | 24.9 ± 2.8 | 92 ± 4 |
| C274 | 20.0 ± 0.8 | 59 ± 5 |
| CH271 | 23.4 ± 0.2 | 17 ± 3 |
| H296 | 42.9 ± 1.4 | 58 ± 4 |
| C-CS1 | 28.2 ± 0.6 | 83 ± 4 |
| CH296 | 41.7 ± 0.8 | 89 ± 5 |
| Proteins . | % Adhesion* . | CFU-GM Production-151 . |
|---|---|---|
| BSA | 8.3 ± 2.6 | 67 ± 3 |
| FN | 24.9 ± 2.8 | 92 ± 4 |
| C274 | 20.0 ± 0.8 | 59 ± 5 |
| CH271 | 23.4 ± 0.2 | 17 ± 3 |
| H296 | 42.9 ± 1.4 | 58 ± 4 |
| C-CS1 | 28.2 ± 0.6 | 83 ± 4 |
| CH296 | 41.7 ± 0.8 | 89 ± 5 |
*FITC-labeled EML-C1 cells were incubated at 37°C for 2 hours on the plates precoated with the indicated proteins, and the proportions of adherent cells were measured by a fluorescence spectrophotometer. Adhesion (%) was calculated as described in Materials and Methods. The results are shown as mean ± SD of triplicate cultures.
EML-C1 cells preincubated with the indicated proteins were plated at 1.0 × 103/mL in methylcellulose media containing SCF + IL-3, and the numbers of CFU-GM were assessed on day 7 of culture. The results (the numbers of GM colonies/1 × 103 EML-C1 cells) are expressed as mean ± SD of quardricate cultures.