Serial In Vitro Assays of Fetal Liver Hematopoietic Progenitors
| Genotype . | In Vitro Passage* . | |||||
|---|---|---|---|---|---|---|
| 1° . | 2° . | 4° . | 6° . | 8° . | 10° . | |
| Wild-type | 198 ± 25† | 2,112 ± 219 | 2,533 ± 525 | 13 ± 19 | 0 | 0 |
| AML1-ETO/+ | 135 ± 60 | 900 ± 154 | 1,321 ± 407 | 702 ± 278 | 600 ± 188 | 650 ± 153 |
| Genotype . | In Vitro Passage* . | |||||
|---|---|---|---|---|---|---|
| 1° . | 2° . | 4° . | 6° . | 8° . | 10° . | |
| Wild-type | 198 ± 25† | 2,112 ± 219 | 2,533 ± 525 | 13 ± 19 | 0 | 0 |
| AML1-ETO/+ | 135 ± 60 | 900 ± 154 | 1,321 ± 407 | 702 ± 278 | 600 ± 188 | 650 ± 153 |
*Hematopoietic cells from methylcellulose cultures were disaggregated, washed, and 3 × 104 viable cells were replated in triplicate into fresh 35-mm methylocellulose cultures.
Numbers represent myeloid and mixed colonies per 3 × 104 viable cells ± SD.