5′ to 3′ Sequence of PCR Primers and Probes
| . | Primer . | Sequence 5′ to >3′ . | EMBL Bank Accession Number . |
|---|---|---|---|
| TGase II | Upper | CAGTTCCAGTTCGTGCCATCA | M55153 |
| Lower | ATGCCTGTCTCCTCCTTCTCG | ||
| Internal | ATCACCCACACCTACAAATACCCAGAG | ||
| β-actin | Upper | ATCATGTTTGAGACCTTCAA | X00351 |
| Lower | CATCTCTTGCTCGAAGTCCA | ||
| Internal | GACCTGGCTGGGCCGGACCTGACTGACTAC | ||
| RAR α | Upper | CATTGAGACCCAGAGCAGC | X006614 |
| Lower | CCGTCTCCGCATCATCCATC | ||
| Internal | GCTCTGAGAGCTACACGCT | ||
| RAR β | Upper | GAATTGAAACACAGAGCACC | Y00291 |
| Lower | GCAGGAGTGGTGACTGACTG | ||
| Internal | GACAGCTGAGTTGGACGATC | ||
| RAR γ | Upper | TGGAGACACAGAGCACCAGCTCA | M38258 |
| Lower | GTCAGTCTGCTGCCTGAAGCC | ||
| Internal | TGAGCCCTCAGTTAGAAGAGC | ||
| RXR α | Upper | CTCCTCAGGCAAGCACTATG | X52773 |
| Lower | AGAGCTTAGCGAACCTTCCC | ||
| Internal | AGGAGCGGCAGCGTGGCAAGGAC | ||
| RXR β | Upper | TCAGGCAAACACTACGGGGT | X63522 |
| Lower | GCATACACTTTCTCCCGCAG | ||
| Internal | AAAGGACAAGGATGGGGATGGGGAG | ||
| RXR γ | Upper | CTCAGGAAAGCACTACGGGG | U38480 |
| Lower | CAGGGTCATTTGTCGAGTTC | ||
| Internal | AGAAGAAAGACAGAGGAGCCGAGAG |
| . | Primer . | Sequence 5′ to >3′ . | EMBL Bank Accession Number . |
|---|---|---|---|
| TGase II | Upper | CAGTTCCAGTTCGTGCCATCA | M55153 |
| Lower | ATGCCTGTCTCCTCCTTCTCG | ||
| Internal | ATCACCCACACCTACAAATACCCAGAG | ||
| β-actin | Upper | ATCATGTTTGAGACCTTCAA | X00351 |
| Lower | CATCTCTTGCTCGAAGTCCA | ||
| Internal | GACCTGGCTGGGCCGGACCTGACTGACTAC | ||
| RAR α | Upper | CATTGAGACCCAGAGCAGC | X006614 |
| Lower | CCGTCTCCGCATCATCCATC | ||
| Internal | GCTCTGAGAGCTACACGCT | ||
| RAR β | Upper | GAATTGAAACACAGAGCACC | Y00291 |
| Lower | GCAGGAGTGGTGACTGACTG | ||
| Internal | GACAGCTGAGTTGGACGATC | ||
| RAR γ | Upper | TGGAGACACAGAGCACCAGCTCA | M38258 |
| Lower | GTCAGTCTGCTGCCTGAAGCC | ||
| Internal | TGAGCCCTCAGTTAGAAGAGC | ||
| RXR α | Upper | CTCCTCAGGCAAGCACTATG | X52773 |
| Lower | AGAGCTTAGCGAACCTTCCC | ||
| Internal | AGGAGCGGCAGCGTGGCAAGGAC | ||
| RXR β | Upper | TCAGGCAAACACTACGGGGT | X63522 |
| Lower | GCATACACTTTCTCCCGCAG | ||
| Internal | AAAGGACAAGGATGGGGATGGGGAG | ||
| RXR γ | Upper | CTCAGGAAAGCACTACGGGG | U38480 |
| Lower | CAGGGTCATTTGTCGAGTTC | ||
| Internal | AGAAGAAAGACAGAGGAGCCGAGAG |
TGase II primers were the same as reported.25