Clonogenicity of Primary GM Colonies
| Cell Source . | Percent Primary Colonies Containing . | No. Clones Generated per Primary Colony. . | ||
|---|---|---|---|---|
| . | Clonogenic Cells . | Median (range) . | ||
| . | Secondary Clones . | Secondary Colonies . | Secondary Clones . | Secondary Colonies . |
| BM, baseline | 49 | 27 | 17 (2-≥300) | 4 (1-89) |
| (n = 338 primary colonies) | 56 | 28 | 30 (2-≥300) | 5 (1-76) |
| 58 | 22 | 3 (2-210) | 2 (1-45) | |
| 73 | 47 | 5 (2-86) | 3 (1-37) | |
| Mean | 59 | 31 | ||
| G-CSF, d5 | 41 | 19 | 8 (2-83) | 2 (1-27) |
| (n = 383 primary colonies) | 48 | 31 | 4 (2-49) | 3 (1-32) |
| 63 | 34 | 24 (2-≥300) | 4 (1-49) | |
| 68 | 31 | 37 (2-169) | 3 (1-23) | |
| Mean | 55 | 29 | ||
| SCF + G-CSF, d5 | 34 | 14 | 5 (2-96) | 2 (1-11) |
| (n = 465 primary colonies) | 38 | 28 | 9 (2-68) | 2 (1-18) |
| 72 | 58 | 53 (2-≥300) | 9 (1-143) | |
| 75 | 33 | 15 (2-165) | 3 (1-130) | |
| Mean | 55 | 33 | ||
| SCF pretreatment, d5 | 87 | 44 | 63 (2-≥300) | 5 (1-103) |
| (n = 336 primary colonies) | 87 | 69 | 26 (2-≥300) | 5 (1-179) |
| 94 | 69 | 85 (2-≥300) | 4 (1-130) | |
| 94 | 86 | 105 (2-≥300) | 23 (1-223) | |
| Mean | 91 | 67 | ||
| Cell Source . | Percent Primary Colonies Containing . | No. Clones Generated per Primary Colony. . | ||
|---|---|---|---|---|
| . | Clonogenic Cells . | Median (range) . | ||
| . | Secondary Clones . | Secondary Colonies . | Secondary Clones . | Secondary Colonies . |
| BM, baseline | 49 | 27 | 17 (2-≥300) | 4 (1-89) |
| (n = 338 primary colonies) | 56 | 28 | 30 (2-≥300) | 5 (1-76) |
| 58 | 22 | 3 (2-210) | 2 (1-45) | |
| 73 | 47 | 5 (2-86) | 3 (1-37) | |
| Mean | 59 | 31 | ||
| G-CSF, d5 | 41 | 19 | 8 (2-83) | 2 (1-27) |
| (n = 383 primary colonies) | 48 | 31 | 4 (2-49) | 3 (1-32) |
| 63 | 34 | 24 (2-≥300) | 4 (1-49) | |
| 68 | 31 | 37 (2-169) | 3 (1-23) | |
| Mean | 55 | 29 | ||
| SCF + G-CSF, d5 | 34 | 14 | 5 (2-96) | 2 (1-11) |
| (n = 465 primary colonies) | 38 | 28 | 9 (2-68) | 2 (1-18) |
| 72 | 58 | 53 (2-≥300) | 9 (1-143) | |
| 75 | 33 | 15 (2-165) | 3 (1-130) | |
| Mean | 55 | 33 | ||
| SCF pretreatment, d5 | 87 | 44 | 63 (2-≥300) | 5 (1-103) |
| (n = 336 primary colonies) | 87 | 69 | 26 (2-≥300) | 5 (1-179) |
| 94 | 69 | 85 (2-≥300) | 4 (1-130) | |
| 94 | 86 | 105 (2-≥300) | 23 (1-223) | |
| Mean | 91 | 67 | ||
GM colonies were grown for 14 days in primary agar cultures, single colonies isolated and recultured for a further 14 days. The number of secondary clones (and colonies) was enumerated and is shown as a percentage. The cell source was bone marrow (BM) taken at baseline or blood taken at day 5 relative to G-CSF administration. Blood was taken after patients received either G-CSF alone, SCF 10 μg/kg/d plus G-CSF for 5 days, or SCF pretreatment. To determine the number of clonogenic cells a mean of 89 colonies was recloned on each occasion (with a mean of 57 colonies generating secondary clones). The number of clones (and colonies) generated per primary colony is also shown. Results were obtained from 4 different patients for each cohort.