Apoptotic Threshold for Cell Lines Exposed to DT388–GM-CSF
| Cell Line . | IC50 (1 × 10−13 mol/L) . | Ratio PSI IC50 /Apopt.IC50 . |
|---|---|---|
| U937 | 10 | 3 |
| TF1 | 20 | 2 |
| HL60neo | 50 | 3 |
| HL60 | 50 | 1 |
| KG1 | 80 | 1 |
| HL60Bcl2 | 500 | 25 |
| THP-1 | 240 | 3.5 |
| HL60BclXL | 1,500 | 150 |
| HL60/VCR | 4,000 | 30 |
| AML193 | 4,000 | 1.3 |
| CEM | >40,000 | ND |
| HEL92.1.7 | >40,000 | ND |
| Cell Line . | IC50 (1 × 10−13 mol/L) . | Ratio PSI IC50 /Apopt.IC50 . |
|---|---|---|
| U937 | 10 | 3 |
| TF1 | 20 | 2 |
| HL60neo | 50 | 3 |
| HL60 | 50 | 1 |
| KG1 | 80 | 1 |
| HL60Bcl2 | 500 | 25 |
| THP-1 | 240 | 3.5 |
| HL60BclXL | 1,500 | 150 |
| HL60/VCR | 4,000 | 30 |
| AML193 | 4,000 | 1.3 |
| CEM | >40,000 | ND |
| HEL92.1.7 | >40,000 | ND |
Assays performed as described in text on at least two separate times. The cell line data were divided into two groups: those with and without elevated anti-apoptotic protein concentrations. A contingency table was then established using ratios less than 4 and greater than or equal to 4. Using Fisher's exact test the probability of a result as extreme as observed under the null hypothesis that elevated and nonelevated ratios come from the same population is less than 0.01.
Abbreviation: ND, not determined.