Numbers of Idiotype- and Isotype-Stimulated IFN-γ– and IL-4–Secreting Cells at Different Time Points From Start of Immunization in Myeloma Patients Immunized With the Autologous Idiotype
| Patient No. . | Weeks . | Unmaintained Follow-up . | |||
|---|---|---|---|---|---|
| 0* . | 4 . | 8 . | 16 . | ||
| IFN-γ–secreting cells/105 PBMC | |||||
| 1: Idiotype | 9 | 31 | 22 | 10 | 20 (w20)-151 |
| Isotype | 1 | 2 | 3 | 4 | 4 |
| 2: Idiotype | 4 | 10 | 21 | 36 | 15 (w65) |
| Isotype | 1 | 2 | 2 | 11 | 2 |
| 3: Idiotype | 5 | 27 | 15 | 7 | 23 (w28) |
| Isotype | 2 | 8 | 2 | 2 | ND |
| 4: Idiotype | 14 | 17 | 16 | 31 | 17 (w24) |
| Isotype | 2 | 4 | 2 | 2 | 10 |
| 5: Idiotype | 6 | 53 | 18 | 40 | 25 (w26) |
| Isotype | 4 | 6 | 7 | 9 | 3 |
| IL-4–secreting cells/105 PBMC | |||||
| 1: Idiotype | 4 | 10 | 2 | 9 | 6 (w20) |
| Isotype | 2 | 1 | 0 | 1 | 1 |
| 2: Idiotype | 1 | 1 | 4 | 1 | 1 (w65) |
| Isotype | 0 | 1 | 2 | 0 | 0 |
| 3: Idiotype | 6 | 2 | 3 | 5 | 3 (w28) |
| Isotype | 3 | 6 | 1 | 2 | ND |
| 4: Idiotype | 4 | 4 | 4 | 14 | 19 (w27) |
| Isotype | 1 | 3 | 2 | 9 | 7 |
| 5: Idiotype | 4 | 6 | 5 | 15 | 16 (w26) |
| Isotype | 1 | 3 | 0 | 2 | 1 |
| Patient No. . | Weeks . | Unmaintained Follow-up . | |||
|---|---|---|---|---|---|
| 0* . | 4 . | 8 . | 16 . | ||
| IFN-γ–secreting cells/105 PBMC | |||||
| 1: Idiotype | 9 | 31 | 22 | 10 | 20 (w20)-151 |
| Isotype | 1 | 2 | 3 | 4 | 4 |
| 2: Idiotype | 4 | 10 | 21 | 36 | 15 (w65) |
| Isotype | 1 | 2 | 2 | 11 | 2 |
| 3: Idiotype | 5 | 27 | 15 | 7 | 23 (w28) |
| Isotype | 2 | 8 | 2 | 2 | ND |
| 4: Idiotype | 14 | 17 | 16 | 31 | 17 (w24) |
| Isotype | 2 | 4 | 2 | 2 | 10 |
| 5: Idiotype | 6 | 53 | 18 | 40 | 25 (w26) |
| Isotype | 4 | 6 | 7 | 9 | 3 |
| IL-4–secreting cells/105 PBMC | |||||
| 1: Idiotype | 4 | 10 | 2 | 9 | 6 (w20) |
| Isotype | 2 | 1 | 0 | 1 | 1 |
| 2: Idiotype | 1 | 1 | 4 | 1 | 1 (w65) |
| Isotype | 0 | 1 | 2 | 0 | 0 |
| 3: Idiotype | 6 | 2 | 3 | 5 | 3 (w28) |
| Isotype | 3 | 6 | 1 | 2 | ND |
| 4: Idiotype | 4 | 4 | 4 | 14 | 19 (w27) |
| Isotype | 1 | 3 | 2 | 9 | 7 |
| 5: Idiotype | 4 | 6 | 5 | 15 | 16 (w26) |
| Isotype | 1 | 3 | 0 | 2 | 1 |
*Mean of three analyses done at three different pretreatment time points.
Week from start of immunization.
Abbreviations: ND, not done; w, week.