Phenotype of S and L Cells
| Protein Detected . | Sorted-Cytospin . | Plated Overnight . | ||
|---|---|---|---|---|
| . | S . | L . | S . | L . |
| Osteopontin | − | +++ (60%) | +++ (90%) | +++ (70%) |
| Collagen-I | − | ++ (50%) | +++ (40%) | +++ (60%) |
| Collagen-II | − | ++ (70%) | +++ (60%) | +++ (80%) |
| Collagen-III | ± (3%) | + (20%) | ++ (25%) | + (40%) |
| CD-44 | − | − | +++ (40%) | ++ (40%) |
| Protein Detected . | Sorted-Cytospin . | Plated Overnight . | ||
|---|---|---|---|---|
| . | S . | L . | S . | L . |
| Osteopontin | − | +++ (60%) | +++ (90%) | +++ (70%) |
| Collagen-I | − | ++ (50%) | +++ (40%) | +++ (60%) |
| Collagen-II | − | ++ (70%) | +++ (60%) | +++ (80%) |
| Collagen-III | ± (3%) | + (20%) | ++ (25%) | + (40%) |
| CD-44 | − | − | +++ (40%) | ++ (40%) |
Immunostaining of cells that were sorted and analyzed (sorted-cytospin) or sorted and plated overnight from S- and L-cell subpopulations. Fluorescence intensity given estimate of level of expression: − none, + low, ++ medium, +++ high, ++++ very high; () indicates percentage of cells expressing the specific phenotypic marker. DAPI staining was used to show present of viable cells in each population; irrelevant antibody and 2nd Ab alone was used to show the absence of nonspecific staining.