RT-PCR Analysis of Gene Expressions in the Pre-B Cells Derived from CD34+ EB Cells
| Primers . | cDNA . | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Spleen* . | Pre-B Cells . | LAK Cells . | Controls . | ||||||||
| ES . | ad† EB34 . | Non-ad‡ EB34 . | Spleen2-153 . | 34a-1 . | 34a-2 . | 34a-3 . | p815 . | 32Dcl3 . | OP9 . | ||
| VpreB | −2-155 | + | + | + | − | − | − | − | − | − | − |
| Ig-β | + | + | + | + | +/− | − | − | − | − | +/− | − |
| VHDJH-Cμ | + | + | +/− | + | − | − | − | − | − | − | − |
| Rag-1 | − | + | +/− | + | − | − | − | − | − | − | − |
| Rag-2 | − | + | +/− | + | − | − | − | − | − | − | − |
| β-Actin | + | + | + | + | + | + | + | + | + | + | + |
| Primers . | cDNA . | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Spleen* . | Pre-B Cells . | LAK Cells . | Controls . | ||||||||
| ES . | ad† EB34 . | Non-ad‡ EB34 . | Spleen2-153 . | 34a-1 . | 34a-2 . | 34a-3 . | p815 . | 32Dcl3 . | OP9 . | ||
| VpreB | −2-155 | + | + | + | − | − | − | − | − | − | − |
| Ig-β | + | + | + | + | +/− | − | − | − | − | +/− | − |
| VHDJH-Cμ | + | + | +/− | + | − | − | − | − | − | − | − |
| Rag-1 | − | + | +/− | + | − | − | − | − | − | − | − |
| Rag-2 | − | + | +/− | + | − | − | − | − | − | − | − |
| β-Actin | + | + | + | + | + | + | + | + | + | + | + |
*Balb/c mouse-derived spleen cells.
Adherent cells, cells grown under the OP9 stroma layer.
Nonadherent cells, cells grown in medium and cells loosely attached to the surface of OP9 cells.
C57BL/6 mouse-derived spleen LAK cells.
Expression levels are semiquantitatively displayed relative to the ES-pre-B signals as follows: +, high level; +/−, low level; −, no expression.