EGFP Expression in Individual GM-CFU Methylcellulose Colonies and Day 13 CFU-S Derived From Mock, Unsorted, EGFP-Negative, and EGFP-Positive Sorted Murine BM Cells After Retroviral Transduction
| Cells . | No. EGFP-Positive GM-CFU/Total No. GM-CFU Analyzed (%) . | No. EGFP-Positive CFU-S/Total No. CFU-S Analyzed* (%) . | ||
|---|---|---|---|---|
| . | . | . | . | . |
| Total mock | 0/39 | (0) | 0/30 | (0) |
| Total EGFP | 29/73 | (40) | 38/50 | (76) |
| EGFP-negative sorted | 1/19 | (5) | 5/20 | (25) |
| EGFP-positive sorted | 24/25 | (96) | 17/20 | (85) |
| Cells . | No. EGFP-Positive GM-CFU/Total No. GM-CFU Analyzed (%) . | No. EGFP-Positive CFU-S/Total No. CFU-S Analyzed* (%) . | ||
|---|---|---|---|---|
| . | . | . | . | . |
| Total mock | 0/39 | (0) | 0/30 | (0) |
| Total EGFP | 29/73 | (40) | 38/50 | (76) |
| EGFP-negative sorted | 1/19 | (5) | 5/20 | (25) |
| EGFP-positive sorted | 24/25 | (96) | 17/20 | (85) |
Individual GM-CFU or day 13 CFU-S derived from the appropriate marrow fractions were isolated and analyzed for EGFP expression by flow cytometry as described in Materials and Methods. The cumulative results for four (GM-CFU) or two (CFU-S) independent experiments are shown.
A CFU-S was determined to be EGFP-positive if >10% of the resuspended cells displayed a distinct green fluorescent signal.