Table 1. Inactivation of T Cells in... | American Society of Hematology

Table 1.

Inactivation of T Cells in Platelet Concentrates by Photochemical Treatment With 150 μmol/L S-59 and UVA Light (N = 4)

Replicate	Number of Leukocytes per mL^*	T-Cell Frequency		Log₁₀-Reduction of Viable T Cells^-153
Replicate	Number of Leukocytes per mL^*	f_control^-151	f_treated^-152	Log₁₀-Reduction of Viable T Cells^-153
1	1.5 × 10⁶	1/22	<1/1.1 × 10⁷	>5.70
2	1.3 × 10⁶	1/41	<1/1.1 × 10⁷	>5.43
3	4.8 × 10⁵	1/43	<1/1.1 × 10⁷	>5.41
4	3.4 × 10⁶	1/110	<1/1.1 × 10⁷	>5.00
Mean ± SD	1.7 ± 1.2 × 10⁶	1/39 ± 1/67	<1/1.1 × 10⁷	>5.39 ± 0.29

Replicate	Number of Leukocytes per mL^*	T-Cell Frequency		Log₁₀-Reduction of Viable T Cells^-153
Replicate	Number of Leukocytes per mL^*	f_control^-151	f_treated^-152	Log₁₀-Reduction of Viable T Cells^-153
1	1.5 × 10⁶	1/22	<1/1.1 × 10⁷	>5.70
2	1.3 × 10⁶	1/41	<1/1.1 × 10⁷	>5.43
3	4.8 × 10⁵	1/43	<1/1.1 × 10⁷	>5.41
4	3.4 × 10⁶	1/110	<1/1.1 × 10⁷	>5.00
Mean ± SD	1.7 ± 1.2 × 10⁶	1/39 ± 1/67	<1/1.1 × 10⁷	>5.39 ± 0.29

*PBMCs isolated from whole blood were spiked into each of four single donor plateletpheresis units in 300 mL of 35% autologous plasma and 65% PASIII to obtain final leukocyte counts that ranged between 4.8 × 10⁵ and 3.4 × 10⁶ per mL. An aliquot was withdrawn from each unit for measurement of the control viable T-cell frequency by the LDA assay as described in Materials and Methods. S-59 was then added to a final concentration of 150 μmol/L and the platelet units illuminated with 1 or 3 Joules/cm²UVA. Aliquots were withdrawn after each dose of UVA for measurement of the treated viable T-cell frequency by LDA. N = 4.

F0-151

f_control = control viable T-cell frequency.

F0-152

f_treated = treated viable T-cell frequency.

F0-153

The log₁₀-reduction is calculated as log₁₀(f_control/f_treated).

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