Detection of Leukemic CFC in the BM of Mice Transplanted With Chronic Phase CML Cells
| Patient No. . | Cell Source . | Time of Analysis (d) . | No. of Mice . | Metaphase Analysis . | |
|---|---|---|---|---|---|
| No. of Ph+ Colonies/ No. of Colonies Analyzed (%) . | |||||
| 2 | BM | 14 | 1 | 1/1 (100) | |
| PB | 17 | 1 | 4/4 (100) | ||
| 4 | BM | 19 | 1 | 20/25 (80) | |
| PB | 10 | 1 | 3/6 (50) | ||
| 5 | PB* | 33 | 1 | 4/4 (100) | |
| 6 | BM | 16 | 1 | 10/16 (62.5) | |
| PB | 21 | 1 | 3/8 (37.5) | ||
| 7 | PB | 46-50 | 5 | 6/10 (60) | |
| 10 | BM | 29-51 | 5 | 18/35 (51.4) | |
| Mean ± SEM | 71 ± 8% | ||||
| Patient No. . | Cell Source . | Time of Analysis (d) . | No. of Mice . | Metaphase Analysis . | |
|---|---|---|---|---|---|
| No. of Ph+ Colonies/ No. of Colonies Analyzed (%) . | |||||
| 2 | BM | 14 | 1 | 1/1 (100) | |
| PB | 17 | 1 | 4/4 (100) | ||
| 4 | BM | 19 | 1 | 20/25 (80) | |
| PB | 10 | 1 | 3/6 (50) | ||
| 5 | PB* | 33 | 1 | 4/4 (100) | |
| 6 | BM | 16 | 1 | 10/16 (62.5) | |
| PB | 21 | 1 | 3/8 (37.5) | ||
| 7 | PB | 46-50 | 5 | 6/10 (60) | |
| 10 | BM | 29-51 | 5 | 18/35 (51.4) | |
| Mean ± SEM | 71 ± 8% | ||||
*Human LTC-IC from this mouse were also analyzed and found to be predominantly normal (15 of 17 CFC progeny generated cytogenetically normal colonies); however, 2 of 17 were Ph+.