NK Function in Normal Dogs and in Four Dogs With DLA-Nonidentical, Unrelated Hematopoietic Grafts
| % of Specific Lysis of Target Cells . | ||||||
|---|---|---|---|---|---|---|
| Effector:Target Cell Ratio . | Normal Dogs (n = 4) . | Allograft Recipients (Dog No.) . | ||||
| Range . | Median . | D823 . | D988 . | E071 . | E072 . | |
| 80:1 | 21.0-29.2 | 24.2 | 29.1 | 32.7 | 23.6 | 8.3 |
| 40:1 | 18.5-32.4 | 20.4 | 24.1 | 27.8 | 25 | 13.2 |
| 20:1 | 13.2-30.8 | 15.5 | 14.7 | 17 | 11.9 | 15.8 |
| 10:1 | 6.7-22.8 | 8.3 | 9.6 | 10.7 | 11.7 | 14.5 |
| 5:1 | 5.0-13.6 | 7.4 | 9.8 | 7.4 | 3.3 | 7.6 |
| % of Specific Lysis of Target Cells . | ||||||
|---|---|---|---|---|---|---|
| Effector:Target Cell Ratio . | Normal Dogs (n = 4) . | Allograft Recipients (Dog No.) . | ||||
| Range . | Median . | D823 . | D988 . | E071 . | E072 . | |
| 80:1 | 21.0-29.2 | 24.2 | 29.1 | 32.7 | 23.6 | 8.3 |
| 40:1 | 18.5-32.4 | 20.4 | 24.1 | 27.8 | 25 | 13.2 |
| 20:1 | 13.2-30.8 | 15.5 | 14.7 | 17 | 11.9 | 15.8 |
| 10:1 | 6.7-22.8 | 8.3 | 9.6 | 10.7 | 11.7 | 14.5 |
| 5:1 | 5.0-13.6 | 7.4 | 9.8 | 7.4 | 3.3 | 7.6 |
51Cr labeled CTAC20 were used as the target cells to quantitate NK activity in four normal dogs and four allograft recipients between 80 to 120 days after transplant. Allograft recipients were treated with MMF and CSP from day 0 to 27. The incubation time for the assay was 15 hours. The data represent the mean of triplicate wells in the assay.