Sub-G1 DNA Content of BMMC or 32D-Kit Cells Treated With Econazole Plus Factor
. | Sub-G1 Content (%) . | |
---|---|---|
No Econazole . | 8 μmol/L Econazole . | |
BMMC | ||
IL-3 | 25.4 | 20.4 |
SLF | 6.8 | 66.8 |
Factor withdrawal | 61.8 | ND |
32D-Kit | ||
IL-3 | 1.6 | 4.8 |
SLF | 2.3 | 35.0 |
Factor withdrawal | 9.2 | ND |
. | Sub-G1 Content (%) . | |
---|---|---|
No Econazole . | 8 μmol/L Econazole . | |
BMMC | ||
IL-3 | 25.4 | 20.4 |
SLF | 6.8 | 66.8 |
Factor withdrawal | 61.8 | ND |
32D-Kit | ||
IL-3 | 1.6 | 4.8 |
SLF | 2.3 | 35.0 |
Factor withdrawal | 9.2 | ND |
BMMC were incubated with 8 μmol/L econazole plus IL-3 (25% WEHI conditioned media), SLF (500 ng/mL), or no factor for 24 hours, stained with propidium iodide, and analyzed for DNA content. 32D-Kit cells were similarly incubated for 18 hours before analysis.
Abbreviation: ND, not determined.