Cytokine Secretion of Neutrophils
| . | — . | LPS . | LPS + rhIL-10 . |
|---|---|---|---|
| TNF-α (pg/mL) | ND | 39.8 ± 6.6* | 13.4 ± 5.5† |
| IL-1β (pg/mL) | ND | 69.0 ± 28.8* | ND |
| . | — . | LPS . | LPS + rhIL-10 . |
|---|---|---|---|
| TNF-α (pg/mL) | ND | 39.8 ± 6.6* | 13.4 ± 5.5† |
| IL-1β (pg/mL) | ND | 69.0 ± 28.8* | ND |
Isolated neutrophils (1 × 106/mL) from healthy individuals were stimulated with LPS (1 μg/mL) in the presence or absence of rhIL-10 (100 U/mL) for 16 hours. Concentrations of cytokines were measured by specific ELISA. IFN-γ, G-CSF, GM-CSF, and IL-10 were not detectable. The data represent the mean ± SEM for five separate experiments.
Abbreviation: ND, not detectable.
P < .05 without agent v LPS.
P < .05 without rhIL-10 v with rhIL-10.