Activation of Thromb-UK by Intact- or Detergent-Lysed Platelets
| Agonist . | S2444 Activity (ΔA/min) . | ||
|---|---|---|---|
| . | Intact pH 7.4 . | Lysate pH 7.4 . | Lysate pH 6.0 . |
| None | 0.001 | 0.030 | 0.116 |
| Thrombin (2.5 to 10 U/mL) | 0.042 | 0.036 | 0.115 |
| ADP (50 μmol/L) | 0.002 | — | — |
| TRAP (60 μmol/L) | 0.008 | — | — |
| Agonist . | S2444 Activity (ΔA/min) . | ||
|---|---|---|---|
| . | Intact pH 7.4 . | Lysate pH 7.4 . | Lysate pH 6.0 . |
| None | 0.001 | 0.030 | 0.116 |
| Thrombin (2.5 to 10 U/mL) | 0.042 | 0.036 | 0.115 |
| ADP (50 μmol/L) | 0.002 | — | — |
| TRAP (60 μmol/L) | 0.008 | — | — |
Intact platelets (5 × 108/mL) at pH 7.4, or Triton X-100 lysates (of equivalent platelet number) at pH 6.0 or 7.4 were mixed with thromb-UK (0.45 μmol/L) in the absence or presence of the indicated agonist for 1 hour at 37°C after which the platelets were pelleted and the supernatant or lysate assayed for S2444 activity in buffer at pH 8.0.