Molecular Evaluation of Minimal Residual Disease After Lineage-Specific Purging
| Patients . | Disease . | . | Apheresis . | Wasted Cells* . | Purified Stem Cells . | Sensitivity3-151 . |
|---|---|---|---|---|---|---|
| 1 | NHL | t(14;18) BCL2/IgH | + | + | − | 105 |
| 2 | NHL | t(14;18) BCL2/IgH | + | + | − | 105 |
| 3 | preB-ALL | TcRγ (Vγ4-Jγ1.3) | + | + | − | 104 |
| 4 | T-ALL | TcRδ (Vδ1-Jδ1) | − | + | − | 104 |
| 5 | preB-ALL | IgH | + | + | − | 104 |
| Patients . | Disease . | . | Apheresis . | Wasted Cells* . | Purified Stem Cells . | Sensitivity3-151 . |
|---|---|---|---|---|---|---|
| 1 | NHL | t(14;18) BCL2/IgH | + | + | − | 105 |
| 2 | NHL | t(14;18) BCL2/IgH | + | + | − | 105 |
| 3 | preB-ALL | TcRγ (Vγ4-Jγ1.3) | + | + | − | 104 |
| 4 | T-ALL | TcRδ (Vδ1-Jδ1) | − | + | − | 104 |
| 5 | preB-ALL | IgH | + | + | − | 104 |
*Wasted cells were eluted after removal of the D column from the magnetic field of the SuperMACS apparatus.
The sensitivity of the PCR reaction was determined by log dilution of the patient's DNA obtained at diagnosis with DNA from a normal donor.