Effect of DT-GM-CSF on AML Cells in the 3H-TdR Uptake Assay
AML . | FAB . | 3H-TdR Uptake (cpm) . | ID50 (ng/mL) . | |||
---|---|---|---|---|---|---|
. | . | no GF . | GM-CSF . | SCF/IL-3/G-CSF . | Autonomous . | Stimulated . |
. | . | . | . | . | Growth . | Growth . |
A | M2 | 1,300 | 8,200 | 33,500 | 0.1 | 5 |
B | M1 | 1,200 | 9,800 | 35,500 | 0.04 | 0.7 |
C | M1 | 1,600 | 2,000 | 31,400 | >500 | >500 |
AML . | FAB . | 3H-TdR Uptake (cpm) . | ID50 (ng/mL) . | |||
---|---|---|---|---|---|---|
. | . | no GF . | GM-CSF . | SCF/IL-3/G-CSF . | Autonomous . | Stimulated . |
. | . | . | . | . | Growth . | Growth . |
A | M2 | 1,300 | 8,200 | 33,500 | 0.1 | 5 |
B | M1 | 1,200 | 9,800 | 35,500 | 0.04 | 0.7 |
C | M1 | 1,600 | 2,000 | 31,400 | >500 | >500 |
Cells from 3 AML patients diagnosed according to the criteria of the FAB committee were incubated in serum-free medium with or without a mixture of SCF, IL-3, and G-CSF.
The ID50 is the concentration of DT-GM-CSF required for 50% inhibition of the autonomous proliferation or the proliferation under stimulatory conditions.