Table 1. Effect of DT-GM-CSF on AML... | American Society of Hematology

Table 1.

Effect of DT-GM-CSF on AML Cells in the 3H-TdR Uptake Assay

AML	FAB	³H-TdR Uptake (cpm)			ID₅₀(ng/mL)
		no GF	GM-CSF	SCF/IL-3/G-CSF	Autonomous	Stimulated
					Growth	Growth
A	M2	1,300	8,200	33,500	0.1	5
B	M1	1,200	9,800	35,500	0.04	0.7
C	M1	1,600	2,000	31,400	>500	>500

AML	FAB	³H-TdR Uptake (cpm)			ID₅₀(ng/mL)
		no GF	GM-CSF	SCF/IL-3/G-CSF	Autonomous	Stimulated
					Growth	Growth
A	M2	1,300	8,200	33,500	0.1	5
B	M1	1,200	9,800	35,500	0.04	0.7
C	M1	1,600	2,000	31,400	>500	>500

Cells from 3 AML patients diagnosed according to the criteria of the FAB committee were incubated in serum-free medium with or without a mixture of SCF, IL-3, and G-CSF.

The ID₅₀ is the concentration of DT-GM-CSF required for 50% inhibition of the autonomous proliferation or the proliferation under stimulatory conditions.

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