Table 1.

Effect of DT-GM-CSF on AML Cells in the 3H-TdR Uptake Assay

AMLFAB3H-TdR Uptake (cpm)ID50 (ng/mL)
no GFGM-CSFSCF/IL-3/G-CSFAutonomousStimulated
GrowthGrowth
M2 1,300 8,200 33,500 0.1 
M1 1,200 9,800 35,500 0.04 0.7 
M1 1,600 2,000 31,400 >500 >500 
AMLFAB3H-TdR Uptake (cpm)ID50 (ng/mL)
no GFGM-CSFSCF/IL-3/G-CSFAutonomousStimulated
GrowthGrowth
M2 1,300 8,200 33,500 0.1 
M1 1,200 9,800 35,500 0.04 0.7 
M1 1,600 2,000 31,400 >500 >500 

Cells from 3 AML patients diagnosed according to the criteria of the FAB committee were incubated in serum-free medium with or without a mixture of SCF, IL-3, and G-CSF.

The ID50 is the concentration of DT-GM-CSF required for 50% inhibition of the autonomous proliferation or the proliferation under stimulatory conditions.

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