Relative Label Distribution of Platelet-Specific Markers in Cultured Megakaryocytes
| . | Golgi/TGR . | End . | MVB I . | MVB II . | α . | PM/DMS . |
|---|---|---|---|---|---|---|
| vWF | 8.8 | 3.6 | 28.6 | 24.2 | 34.8 | ND |
| β-TG | 26.7 | 5.1 | 11.1 | 11.8 | 45.3 | ND |
| GPIIb-IIIa | 4.8 | 18.4 | 7.1 | 3.8 | 40.4 | 25.5 |
| P-selectin | 23 | 9.4 | 16.8 | 25.2 | 22.9 | 2.7 |
| CD63 | 1.5 | 14.1 | 43.2 | 39 | 2.2 | ND |
| . | Golgi/TGR . | End . | MVB I . | MVB II . | α . | PM/DMS . |
|---|---|---|---|---|---|---|
| vWF | 8.8 | 3.6 | 28.6 | 24.2 | 34.8 | ND |
| β-TG | 26.7 | 5.1 | 11.1 | 11.8 | 45.3 | ND |
| GPIIb-IIIa | 4.8 | 18.4 | 7.1 | 3.8 | 40.4 | 25.5 |
| P-selectin | 23 | 9.4 | 16.8 | 25.2 | 22.9 | 2.7 |
| CD63 | 1.5 | 14.1 | 43.2 | 39 | 2.2 | ND |
Gold particles were counted over cryosections of cultured megakaryocytes after immunolabeling as indicated. The gold particles were attributed to the selected compartments indicated at the top. The data are expressed as percentages of the total number of gold particles counted over the selected structures.
Abbreviations: PM, plasma membrane; DMS, demarcating membrane system; End, tubulo-vesicular structures reminiscent of endosomes; ND, not determined.