Factor Dependency of Osteoclastogenesis in the Osteoclast Culture Period
| Factor Added* . | No. of TRAP+ Multinucleated Cells (TRAP+ Mononucleated Cells)/Well† . | |||
|---|---|---|---|---|
| M-CSF . | 1α,25-(OH)2D3 . | Dex . | OP9 . | ST2 . |
| + | + | + | 20.3 ± 2.1 (65.3 ± 8.3) | ND |
| + | + | − | 0 (0) | ND |
| + | − | + | 0 (0) | ND |
| + | − | − | 0 (0) | ND |
| − | + | + | 0.3 ± 0.6 (3.3 ± 2.3) | 270.7 ± 57.4(NC) |
| − | + | − | 0 (0) | 304.3 ± 79.8(NC) |
| − | − | + | 0 (0) | 0 (1.3 ± 1.5) |
| − | − | − | 0 (0) | 0 (0) |
| Factor Added* . | No. of TRAP+ Multinucleated Cells (TRAP+ Mononucleated Cells)/Well† . | |||
|---|---|---|---|---|
| M-CSF . | 1α,25-(OH)2D3 . | Dex . | OP9 . | ST2 . |
| + | + | + | 20.3 ± 2.1 (65.3 ± 8.3) | ND |
| + | + | − | 0 (0) | ND |
| + | − | + | 0 (0) | ND |
| + | − | − | 0 (0) | ND |
| − | + | + | 0.3 ± 0.6 (3.3 ± 2.3) | 270.7 ± 57.4(NC) |
| − | + | − | 0 (0) | 304.3 ± 79.8(NC) |
| − | − | + | 0 (0) | 0 (1.3 ± 1.5) |
| − | − | − | 0 (0) | 0 (0) |
Abbreviations: ND, not done; NC, not counted.
Osteoclastogenesis of ES-derived cells was examined in the presence or absence of the indicated factors. Cultures were initiated by inoculating 104 cells per well and continued according to the A schedule of Fig 1. On day 11 TRAP assay was performed.
Values are means ± SD of triplicate cultures.