Influence of IgG and F(ab′)2 on the Binding of 125I-GM-CSF to PMN
| Batch . | Competitor . | Competitor . | ||
|---|---|---|---|---|
| None . | GM-CSF . | MoAb to GM-CSF-R . | ||
| None | None | 278 | 17 | 24 |
| 1 | IgG | 230 | 20 | 131 |
| 1 | F(ab′)2 | 97 | 18 | 21 |
| 2 | IgG | 327 | 20 | 284 |
| 2 | F(ab′)2 | 42 | 22 | 21 |
| 3 | IgG | 265 | 21 | 20 |
| 3 | F(ab′)2 | 252 | 19 | 20 |
| Batch . | Competitor . | Competitor . | ||
|---|---|---|---|---|
| None . | GM-CSF . | MoAb to GM-CSF-R . | ||
| None | None | 278 | 17 | 24 |
| 1 | IgG | 230 | 20 | 131 |
| 1 | F(ab′)2 | 97 | 18 | 21 |
| 2 | IgG | 327 | 20 | 284 |
| 2 | F(ab′)2 | 42 | 22 | 21 |
| 3 | IgG | 265 | 21 | 20 |
| 3 | F(ab′)2 | 252 | 19 | 20 |
Three batches of IgG, 3.6 mg/mL, were tested in parallel with the corresponding F(ab′)2 fragments. The results are shown as mean cpmbound (N = 2) to 2 × 106 PMN incubated with 125I-GM-CSF at 2,000 cpm/200 4 μL. Cpmfree in supernatants of samples added only IgG or F(ab′)2 were: batch 1, 232 and 264; batch 2, 125 and 112; batch 3, 1,602 and 1,570.