Quantitation of Native and Recombinant GM-CSF and IL-5 by Different Immunoassays
| Cytokine . | IgG Batch . | Assay . | Native Cytokine (ngeq/mL) . | Recombinant Cytokine (ngeq/ng) . | ||
|---|---|---|---|---|---|---|
| Reference = E coli GM-CSF: | Yeast | CHO | ||||
| GM-CSF | 1 | RIA | 19 | 0.5 | 0.5 | |
| 1 | F/T v. T | 27 | ||||
| 2 | RIA | 19 | 0.3 | 0.03 | ||
| 2 | F/T v. T | 28 | ||||
| 3 | RIA | 20 | 0.3 | 0.1 | ||
| 4 | RIA | 18 | 0.2 | 0.05 | ||
| ELISA | 27 | 0.74 | 0.6 | |||
| Reference = Sf-21 IL-5: | SW 25 | T ni | E coli | |||
| IL-5 | 5 | RIA | 2.4 | 1 | 0.1 | 0.05 |
| 5 | F/T v. T | 2.8 | ||||
| ELISA | 1.6 | 0.66 | 0.1 | 0.04 | ||
| Cytokine . | IgG Batch . | Assay . | Native Cytokine (ngeq/mL) . | Recombinant Cytokine (ngeq/ng) . | ||
|---|---|---|---|---|---|---|
| Reference = E coli GM-CSF: | Yeast | CHO | ||||
| GM-CSF | 1 | RIA | 19 | 0.5 | 0.5 | |
| 1 | F/T v. T | 27 | ||||
| 2 | RIA | 19 | 0.3 | 0.03 | ||
| 2 | F/T v. T | 28 | ||||
| 3 | RIA | 20 | 0.3 | 0.1 | ||
| 4 | RIA | 18 | 0.2 | 0.05 | ||
| ELISA | 27 | 0.74 | 0.6 | |||
| Reference = Sf-21 IL-5: | SW 25 | T ni | E coli | |||
| IL-5 | 5 | RIA | 2.4 | 1 | 0.1 | 0.05 |
| 5 | F/T v. T | 2.8 | ||||
| ELISA | 1.6 | 0.66 | 0.1 | 0.04 | ||
The activities of native and recombinant cytokines were measured by RIA and F/T versus T using different IgG batches and, also, with ELISA using MoAb from the kit manufacturer. Results are mean of triplicates, SDs were <10%.