Complementing Activity and Subcellular Localization of Wild-Type FAA and Constructed Mutants
| Construct* . | Complementation† . | Subcellular Localization . |
|---|---|---|
| pDRFAA | + (41.3 ± 5.2) | Cytoplasm |
| pDRFAA-M116 | − (5.9 ± 1.1) | Cytoplasm |
| pDRmNLSFAA | + (41.9 ± 4.3) | Cytoplasm |
| pDRN271-FAA | − (5.6 ± 2.1) | Cytoplasm |
| pDRFAAL3P | − (6.1 ± 2.4) | Cytoplasm |
| pDRFAAL3, 4V | + (54.1 ± 5.9) | Cytoplasm |
| Construct* . | Complementation† . | Subcellular Localization . |
|---|---|---|
| pDRFAA | + (41.3 ± 5.2) | Cytoplasm |
| pDRFAA-M116 | − (5.9 ± 1.1) | Cytoplasm |
| pDRmNLSFAA | + (41.9 ± 4.3) | Cytoplasm |
| pDRN271-FAA | − (5.6 ± 2.1) | Cytoplasm |
| pDRFAAL3P | − (6.1 ± 2.4) | Cytoplasm |
| pDRFAAL3, 4V | + (54.1 ± 5.9) | Cytoplasm |
Constructs were generated both with and without GFP tag.
The ability of the constructs to complement MMC-hypersensitivity in HSC72 cells is indicated with + and lack of complementation by −. Numbers are IC50 values in nmol/L MMC including standard deviations obtained from at least three independent experiments. Results of mutants with and without GFP tag were similar and taken together for the calculation of averages. Control-transfected HSC72 cells using pDR2 alone had an average IC50 of 6.2 ± 2.2.