Clonogenic Capacity of CD34+lin−Flt3high Versus Flt3low Marrow Cells After Suspension Culture
| Cytokines . | Day 6 . | Day 9 . | ||||||
|---|---|---|---|---|---|---|---|---|
| Flt3high . | Flt3low . | Flt3high . | Flt3low . | |||||
| Clusters . | CFU-C . | Clusters . | CFU-C . | Clusters . | CFU-C . | Clusters . | CFU-C . | |
| FL | ND | ND | ND | ND | 1500 | 1500 | 11 | 8 |
| FL + KL | ND | ND | ND | ND | 21,000 | 840 | 49 | 84 |
| FL + 5 GF* | 868 | 31,640 | 730 | 19,856 | 5,200 | 15,600 | 1,410 | 1,833 |
| Cytokines . | Day 6 . | Day 9 . | ||||||
|---|---|---|---|---|---|---|---|---|
| Flt3high . | Flt3low . | Flt3high . | Flt3low . | |||||
| Clusters . | CFU-C . | Clusters . | CFU-C . | Clusters . | CFU-C . | Clusters . | CFU-C . | |
| FL | ND | ND | ND | ND | 1500 | 1500 | 11 | 8 |
| FL + KL | ND | ND | ND | ND | 21,000 | 840 | 49 | 84 |
| FL + 5 GF* | 868 | 31,640 | 730 | 19,856 | 5,200 | 15,600 | 1,410 | 1,833 |
CD34+lin− marrow cells sorted into Flt3high and Flt3low subpopulations were cultured at a density of 104 cells/well in 24-well dishes in the presence of the designated cytokines at concentrations described in Materials and Methods. On days 6 and 9 of culture, 500 cells from each well were plated in methylcellulose in triplicates. The values shown are the total numbers of clusters and CFU-C (as defined in Material and Methods31) per suspension culture. On day 0 (before the start of the suspension cultures), there were 1,172 CFU-C in the Flt3high and 536 CFU-C in the Flt3lowsubpopulations; blast colonies were not determined.
Abbreviation: ND, Not determined.
GF, growth factors: KL, IL-6, IL-3, GM-CSF, Epo.