mRNA Cytokine Expression in Stromal Cultures of Fetal and Adult Bone Marrow
| Phenotype . | Bone . | Fibronectin . | TPO . | FL . | IL-3 . | SCF . | CD34 . |
|---|---|---|---|---|---|---|---|
| . | Marrow . | . | . | . | . | . | . |
| CD34+, THY-1+ | Fetal | 6/7 | 6/7 | 2/7 | 0/4 | 2/2 | 1/2 |
| CD34−, THY-1+ | Fetal | 4/22 | 20/22 | 2/22 | 0/22 | 0/22 | 0/22 |
| CD34+ | Adult | 5/13 | 4/4 | 2/4 | 0/2 | 0/5 | 0/2 |
| CD34− | Adult | 4/12 | 3/3 | 1/3 | 0/2 | 0/4 | 0/2 |
| Unsorted | Adult | 8/13 | 7/8 | 3/8 | ND | ND | ND |
| Phenotype . | Bone . | Fibronectin . | TPO . | FL . | IL-3 . | SCF . | CD34 . |
|---|---|---|---|---|---|---|---|
| . | Marrow . | . | . | . | . | . | . |
| CD34+, THY-1+ | Fetal | 6/7 | 6/7 | 2/7 | 0/4 | 2/2 | 1/2 |
| CD34−, THY-1+ | Fetal | 4/22 | 20/22 | 2/22 | 0/22 | 0/22 | 0/22 |
| CD34+ | Adult | 5/13 | 4/4 | 2/4 | 0/2 | 0/5 | 0/2 |
| CD34− | Adult | 4/12 | 3/3 | 1/3 | 0/2 | 0/4 | 0/2 |
| Unsorted | Adult | 8/13 | 7/8 | 3/8 | ND | ND | ND |
Twenty-nine stromal cell cultures derived from single FACS-sorted fetal bone marrow cells (F) and 25 stromal cultures that had been initiated by single adult bone marrow stromal cells (A) were analyzed for the presence of cytokine mRNA species. The presence of intact RNA in each sample was confirmed by amplifying the fibronectin (stromal cell–specific) and/or actin cDNA sequences, using specific primer sequences. The presence of specific PCR products for each gene of interest (TPO, SCF, IL-3, FL, and CD34) was confirmed by Southern blot hybridization using a 32P-labeled internal probe.