Table 2. Cultures of CD34 Stromal and... | American Society of Hematology

Table 2.

Cultures of CD34⁺ Stromal and Hematopoietic Bone Marrow Cells Maintain Colony-Forming Cells in the Absence of Exogenous Cytokines

	BFU-E	CFU-E	CFU-G	CFU-M	CFU-GM	CFU-GEMM	CFU-MEG
Experiment 1	35	26	50	40	23	2	2
Experiment 2	17	46	22	44	3	1	6

	BFU-E	CFU-E	CFU-G	CFU-M	CFU-GM	CFU-GEMM	CFU-MEG
Experiment 1	35	26	50	40	23	2	2
Experiment 2	17	46	22	44	3	1	6

Nonadherent cells were gently aspirated from mixed hematopoietic/stromal cultures that had been initiated with CD34⁺ cells from adult bone marrow samples. Nonadherent cells plus media were centrifuged at 1,300 rpm for 10 minutes, resuspended in 1 mL PBS + 5% FBS, and counted. For methylcellulose cultures and plasma clot assay, 3 × 10⁵ CD34⁺ nonadherent cells were plated in triplicate wells. CFC activity in methylcellulose was counted after 2 weeks of culture; the number of CFU-MEG was counted after 8 days of culture.

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