Poisson Analysis of the Relative Frequency and Absolute Number of CD34+ and CD34− Stromal Progenitors per 1 × 106 Adult Bone Marrow Mononuclear Cells
| . | Bone Marrow . | CD34+ . | CD34− . | CDw90+, . | CDw90−, . |
|---|---|---|---|---|---|
| . | Mononuclear Cells . | . | . | CD34− . | CD34− . |
| Median frequency in BM | 1/4,000 | 1/5,000 | 1/4,500 | 1/500 | 1/200,000 |
| Stromal progenitors per 1 × 106 BM cells | 250 | 2 | 200 | ND | ND |
| n = 22 | n = 22 | n = 22 | n = 5 | n = 5 |
| . | Bone Marrow . | CD34+ . | CD34− . | CDw90+, . | CDw90−, . |
|---|---|---|---|---|---|
| . | Mononuclear Cells . | . | . | CD34− . | CD34− . |
| Median frequency in BM | 1/4,000 | 1/5,000 | 1/4,500 | 1/500 | 1/200,000 |
| Stromal progenitors per 1 × 106 BM cells | 250 | 2 | 200 | ND | ND |
| n = 22 | n = 22 | n = 22 | n = 5 | n = 5 |
CD34+ and CD34− bone marrow subset cells, selected using immunomagnetic beads, were plated at a series of concentrations from 5 × 104 to 1 × 102 cells/well into 8 to 24 wells in a 96-well tissue culture plate containing 200 μL tissue culture medium. Each well was scored for the growth of 50 or more fibroblastic cells after 14 to 21 days of culture at 37°C. The frequency of clonogenic stromal cells was determined as the cell concentration at which 37% of the wells showed no stromal growth. ND, not determined.