Oligonucleotide Primers and Internal Probes
| Fibronectin (305 bp) | Sense: 5′TGTCTATGCTCAGAATCCAAGCGG-3′ |
| Antisense: 5′GCTGGCTCTCCATATCATCGTGC-3′ | |
| Internal probe: 5′TAGGGCGATCAATGTTGGTTACTG-3′ | |
| SCF (502 bp) | Sense: 5′GCCGCTGTTCGTGCAATATGC-3′ |
| Antisense: 5′GGAGTAAAGAGCCTGGGTTCTGGG-3′ | |
| Internal probe: 5′TGTGGAGTGCGTCAAAGA-3′ | |
| CD34 (659 bp) | Sense: 5′CAAGGCAGAAATCAAATGTTCAGGC-3′ |
| Antisense: 5′TCACCTAGCCGAGTCACAATTCG-3′ | |
| Internal probe: 5′TGTGGAGTGCGTCAAAGA-3′ | |
| IL-3 (500 bp) | Sense: 5′TGCTGGACTTCAACAACCTCAATGG-3′ |
| Antisense: 5′CAACCGCACAAGGCCAAATGG-3′ | |
| Internal probe: 5′CACCCACGCGACATCCAA-3′ | |
| TPO (347 bp) | Sense: 5′GTAGGGGTGGGCGTTGGAGCAG-3′ |
| Antisense: 5′AAGTGGCAGCAGGGATTCAGAGC-3′ | |
| Internal probe: 5′CCTAGGAGCCCCGGACATTTC-3′ | |
| FL (517 bp) | Sense: 5′CCCCCGGCCGAAATGACAGTG-3′ |
| Antisense: 5′ATGGGGGTGGCAGGGTTGAGGA-3′ | |
| Internal probe: 5′CACAGCCCCGATCTCCGACTTC-3′ |
| Fibronectin (305 bp) | Sense: 5′TGTCTATGCTCAGAATCCAAGCGG-3′ |
| Antisense: 5′GCTGGCTCTCCATATCATCGTGC-3′ | |
| Internal probe: 5′TAGGGCGATCAATGTTGGTTACTG-3′ | |
| SCF (502 bp) | Sense: 5′GCCGCTGTTCGTGCAATATGC-3′ |
| Antisense: 5′GGAGTAAAGAGCCTGGGTTCTGGG-3′ | |
| Internal probe: 5′TGTGGAGTGCGTCAAAGA-3′ | |
| CD34 (659 bp) | Sense: 5′CAAGGCAGAAATCAAATGTTCAGGC-3′ |
| Antisense: 5′TCACCTAGCCGAGTCACAATTCG-3′ | |
| Internal probe: 5′TGTGGAGTGCGTCAAAGA-3′ | |
| IL-3 (500 bp) | Sense: 5′TGCTGGACTTCAACAACCTCAATGG-3′ |
| Antisense: 5′CAACCGCACAAGGCCAAATGG-3′ | |
| Internal probe: 5′CACCCACGCGACATCCAA-3′ | |
| TPO (347 bp) | Sense: 5′GTAGGGGTGGGCGTTGGAGCAG-3′ |
| Antisense: 5′AAGTGGCAGCAGGGATTCAGAGC-3′ | |
| Internal probe: 5′CCTAGGAGCCCCGGACATTTC-3′ | |
| FL (517 bp) | Sense: 5′CCCCCGGCCGAAATGACAGTG-3′ |
| Antisense: 5′ATGGGGGTGGCAGGGTTGAGGA-3′ | |
| Internal probe: 5′CACAGCCCCGATCTCCGACTTC-3′ |
Primer pairs are shown for the amplification of human cytokine messages by PCR and internal primers used as probes for the hybridization. Sequences are in 5′-3′ orientation.