Contribution (%) of Different Molecular Forms of MMP-2 to the Total MMP-2 Level in Serum-Free Conditioned Medium of Fibroblasts or Smooth Muscle Cells Derived From MMP-3+/+ or MMP-3−/− Mice
| . | Fibroblasts . | Smooth Muscle Cells . | ||
|---|---|---|---|---|
| MMP-3+/+ . | MMP-3−/− . | MMP-3+/+ . | MMP-3−/− . | |
| 70 kD proMMP-2 | ||||
| 24 h | 17 ± 1 | 25 ± 7 | 16 ± 3 | 28 ± 5 |
| 48 h | 20 ± 3 | 24 ± 7 | 32 ± 1 | 33 ± 5 |
| 72 h | 23 ± 7 | 21 ± 1 | 29 ± 4 | 32 ± 5 |
| 65 kD proMMP-2 | ||||
| 24 h | 45 ± 2 | 60 ± 2* | 83 ± 3 | 67 ± 5 |
| 48 h | 48 ± 5 | 56 ± 4 | 75 ± 10 | 58 ± 6 |
| 72 h | 40 ± 8 | 41 ± 9 | 65 ± 6 | 52 ± 5 |
| 58 kD MMP-2 | ||||
| 24 h | 37 ± 1 | 15 ± 6 | 1 ± 1 | 5 ± 0.1 |
| 48 h | 33 ± 3 | 20 ± 5 | 4 ± 1 | 9 ± 1 |
| 72 h | 37 ± 5 | 38 ± 8 | 6 ± 2 | 16 ± 3 |
| . | Fibroblasts . | Smooth Muscle Cells . | ||
|---|---|---|---|---|
| MMP-3+/+ . | MMP-3−/− . | MMP-3+/+ . | MMP-3−/− . | |
| 70 kD proMMP-2 | ||||
| 24 h | 17 ± 1 | 25 ± 7 | 16 ± 3 | 28 ± 5 |
| 48 h | 20 ± 3 | 24 ± 7 | 32 ± 1 | 33 ± 5 |
| 72 h | 23 ± 7 | 21 ± 1 | 29 ± 4 | 32 ± 5 |
| 65 kD proMMP-2 | ||||
| 24 h | 45 ± 2 | 60 ± 2* | 83 ± 3 | 67 ± 5 |
| 48 h | 48 ± 5 | 56 ± 4 | 75 ± 10 | 58 ± 6 |
| 72 h | 40 ± 8 | 41 ± 9 | 65 ± 6 | 52 ± 5 |
| 58 kD MMP-2 | ||||
| 24 h | 37 ± 1 | 15 ± 6 | 1 ± 1 | 5 ± 0.1 |
| 48 h | 33 ± 3 | 20 ± 5 | 4 ± 1 | 9 ± 1 |
| 72 h | 37 ± 5 | 38 ± 8 | 6 ± 2 | 16 ± 3 |
Data were obtained by zymography on gelatin-containing gels and are mean ± SEM of 3 experiments.
P < .01 versus MMP-3+/+.