Effects of Annexin V and Oxidation on Phospholipid-supported Thrombin Generation
| Lipid . | Unmodified . | Annexin V . | Oxidation . |
|---|---|---|---|
| Thrombin (nmol/L) (mean ± SEM) | |||
| Phosphatidyl inositol (125 μmol/L) | 741 ± 130 | 259 ± 85† | |
| Phosphatidyl inositol (72 μmol/L) | 204 ± 86 | 382 ± 131† | |
| Phosphatidyl ethanolamine (677 μmol/L) | 965 ± 61 | 460 ± 136‡ | |
| Phosphatidyl ethanolamine (163 μmol/L) | 257 ± 108 | 478 ± 117‡ | |
| Phosphatidyl serine (412 μmol/L) | 973 ± 72 | 425 ± 147‡ | |
| Phosphatidyl serine (270 μmol/L) | 221 ± 71 | 447 ± 158* | |
| Phosphatidyl glycerol (487 μmol/L) | 806 ± 189 | 141 ± 56* | |
| Phosphatidyl glycerol (488 μmol/L) | 821 ± 95 | 552 ± 294 |
| Lipid . | Unmodified . | Annexin V . | Oxidation . |
|---|---|---|---|
| Thrombin (nmol/L) (mean ± SEM) | |||
| Phosphatidyl inositol (125 μmol/L) | 741 ± 130 | 259 ± 85† | |
| Phosphatidyl inositol (72 μmol/L) | 204 ± 86 | 382 ± 131† | |
| Phosphatidyl ethanolamine (677 μmol/L) | 965 ± 61 | 460 ± 136‡ | |
| Phosphatidyl ethanolamine (163 μmol/L) | 257 ± 108 | 478 ± 117‡ | |
| Phosphatidyl serine (412 μmol/L) | 973 ± 72 | 425 ± 147‡ | |
| Phosphatidyl serine (270 μmol/L) | 221 ± 71 | 447 ± 158* | |
| Phosphatidyl glycerol (487 μmol/L) | 806 ± 189 | 141 ± 56* | |
| Phosphatidyl glycerol (488 μmol/L) | 821 ± 95 | 552 ± 294 |
Thrombin generation supported by unmodified phospholipids, phospholipids incubated with annexin V, and phospholipids modified by oxidation. Phospholipid concentrations were chosen to ensure that either reductions (with annexin V) or increases (with oxidation) would be measurable on the thrombin generation standard curve. The presented results were obtained after incubation with 10 μmol/L annexin V (similar results were obtained with 5 μmol/L annexin V) and 2.5 μmol/L cupric sulfate. Comparisons of mean thrombin concentrations, by Student's t-test, were undertaken for phospholipids: (1) incubated with or without annexin V or (2) incubated with and without cupric sulfate.
P = .015.
P = .02.
P = .03 (mean ± SEM, all experiments were undertaken on three to eight separate occasions).