Effects of Annexin V and Modification by Oxidation and Glycosylation on Lipoprotein-supported Thrombin Generation
| Lipoprotein/ Platelets . | Unmodified . | Annexin V . | Oxidation . | Glycosylation . |
|---|---|---|---|---|
| Thrombin (nmol/L) (mean ± SEM) | ||||
| Chylomicrons | 292 ± 60* | 224 ± 115 | ||
| VLDL | 503 ± 58* | 273 ± 73‡ | 542 ± 91 | 499 ± 95 |
| LDL | 43 ± 12 | 526 ± 87† | 94 ± 40 | |
| HDL | 26 ± 11 | 77 ± 46 | ||
| Platelets | 338 ± 50 | 178 ± 18§ |
| Lipoprotein/ Platelets . | Unmodified . | Annexin V . | Oxidation . | Glycosylation . |
|---|---|---|---|---|
| Thrombin (nmol/L) (mean ± SEM) | ||||
| Chylomicrons | 292 ± 60* | 224 ± 115 | ||
| VLDL | 503 ± 58* | 273 ± 73‡ | 542 ± 91 | 499 ± 95 |
| LDL | 43 ± 12 | 526 ± 87† | 94 ± 40 | |
| HDL | 26 ± 11 | 77 ± 46 | ||
| Platelets | 338 ± 50 | 178 ± 18§ |
Thrombin generation supported by unmodified lipoproteins, lipoproteins modified by oxidation and nonenzymatic glycosylation, and lipoproteins incubated with annexin V. Experiments were undertaken with lipoproteins from 12 subjects (mean ± SEM) containing 1 μg of total (apolipo)protein or 1 × 106 platelets in the absence and presence of annexin V. Lipoproteins were oxidized by incubation with 2.5 μmol/L cupric sulfate. Of the lipoproteins, only VLDL was incubated with annexin V (5 μmol/L). Results for nonenzymatic glycosylation of lipoproteins were obtained after incubation with 20 mmol/L glucose (nonenzymatic glycosylation was only undertaken with VLDL and LDL). Specific comparisons between mean thrombin concentrations were undertaken by Student'st-test.
P < .00001 for chylomicrons or VLDL versus HDL.
P < .0001 for oxidized LDL versus unmodified LDL.
P = .002 for VLDL with annexin V versus unmodified VLDL.
P = .015 for platelets with annexin V compared to platelets alone.