Effect of NOK-2 and Fas-Fc on rhIFNγ-Induced ECFC Inhibition
| . | Normal Colonies* . | Apoptotic Colonies† . | ||
|---|---|---|---|---|
| Large . | Medium . | Small . | ||
| Experiment no. 1 | ||||
| Control | 33 ± 2 | 20 ± 3 | 14 ± 2 | 0 |
| rhIFNγ | 16 ± 2 | 40 ± 2 | ||
| rhIFNγ + NOK-2 | 2 ± 1 | 33 ± 3 | 28 ± 3 | 0 |
| rhIFNγ + Fas-Fc | 14 ± 2 | 43 ± 2 | 7 ± 1 | |
| Experiment no. 2 | ||||
| Control | 20 ± 5 | 25 ± 7 | 24 ± 2 | 0 |
| rhIFNγ | 51 ± 4 | |||
| rhIFNγ + NOK-2 | 6 ± 3 | 42 ± 4 | 13 ± 2 | |
| rhIFNγ + Fas-Fc | 2 ± 1 | 27 ± 2 | 28 ± 2 | |
| . | Normal Colonies* . | Apoptotic Colonies† . | ||
|---|---|---|---|---|
| Large . | Medium . | Small . | ||
| Experiment no. 1 | ||||
| Control | 33 ± 2 | 20 ± 3 | 14 ± 2 | 0 |
| rhIFNγ | 16 ± 2 | 40 ± 2 | ||
| rhIFNγ + NOK-2 | 2 ± 1 | 33 ± 3 | 28 ± 3 | 0 |
| rhIFNγ + Fas-Fc | 14 ± 2 | 43 ± 2 | 7 ± 1 | |
| Experiment no. 2 | ||||
| Control | 20 ± 5 | 25 ± 7 | 24 ± 2 | 0 |
| rhIFNγ | 51 ± 4 | |||
| rhIFNγ + NOK-2 | 6 ± 3 | 42 ± 4 | 13 ± 2 | |
| rhIFNγ + Fas-Fc | 2 ± 1 | 27 ± 2 | 28 ± 2 | |
Day-5 cells were cultured in plasma clots with or without rhIFNγ at 250 U/mL and with or without neutralizing anti-FasL MoAb, NOK-2, or Fas-Fc. After incubation for 10 days, the clots were fixed and stained. NOK-2 (20 μg/mL) was added at the start of incubation and 2.5 μg/mL was added at 24 hours and 48 hours of incubation. Fas-Fc (10 μg/mL) was added at the start of incubation and 5 μg/mL was added at 24 hours of incubation. Equal volumes of media were added to the controls.
Erythroid colony number per 100 cells plated; large colonies, greater than 500 cells per colony; medium colonies, 50 to 500 cells per colony; small colonies, 2 to 49 cells per colony.
Colonies with more than 10% apoptotic cells.