Primers Used for VH Gene Amplification
| Name . | Sequence (5′-3′) . |
|---|---|
| VH1FW* | CAGGTTCAGCTGGTGCAGTCTGG |
| VH2FW | CAGGTCACCTTGAAGGAGTCTGG |
| VH3FW | GAGGTGCAGCTGGTGGAGTCTGG |
| VH4aFW | CAGGTGCAGCTGCAGGAGTCGGG |
| VH4bFW | CAGGTGCAGCTACAGCAGTGGGG |
| VH6FW | CAGGTACAGCTGCAGCAGTCAGG |
| VH1FWI | CCTCAGTGAAGGT(C,T)TCCTGCAAGG |
| VH2FWI | CAGACCCTCAC(A,G)CTGACCTGCACC |
| VH3FWI | GGTCCCTGAGACTCTCCTGTGCAG |
| VH4FWI | TCGGAGACCCTGTCCCTCACCTGC |
| VH5FWI | GTGAAAAAGCCCGGGGAGTCTCTG |
| VH6FWI | CTCACTCACCTGTGCCATCTCCGG |
| FW3 | GACACGGC(C,T)(A,G)TGTATTACTG |
| JH1 | ACCTGAGGAGACGGTGACC |
| JH2 | CCA(G,T)GGT(C,G,T)CCTTGGCCCCAG |
| Name . | Sequence (5′-3′) . |
|---|---|
| VH1FW* | CAGGTTCAGCTGGTGCAGTCTGG |
| VH2FW | CAGGTCACCTTGAAGGAGTCTGG |
| VH3FW | GAGGTGCAGCTGGTGGAGTCTGG |
| VH4aFW | CAGGTGCAGCTGCAGGAGTCGGG |
| VH4bFW | CAGGTGCAGCTACAGCAGTGGGG |
| VH6FW | CAGGTACAGCTGCAGCAGTCAGG |
| VH1FWI | CCTCAGTGAAGGT(C,T)TCCTGCAAGG |
| VH2FWI | CAGACCCTCAC(A,G)CTGACCTGCACC |
| VH3FWI | GGTCCCTGAGACTCTCCTGTGCAG |
| VH4FWI | TCGGAGACCCTGTCCCTCACCTGC |
| VH5FWI | GTGAAAAAGCCCGGGGAGTCTCTG |
| VH6FWI | CTCACTCACCTGTGCCATCTCCGG |
| FW3 | GACACGGC(C,T)(A,G)TGTATTACTG |
| JH1 | ACCTGAGGAGACGGTGACC |
| JH2 | CCA(G,T)GGT(C,G,T)CCTTGGCCCCAG |
*Number in VH primer name refers to the VH family. The VH1FW primer will also recognize FW1 region of VH5 family. Primers ending with “I” are specific for VH FW1 regions 3′ (internal) to the others.