Cells Expressing β-gal Activity in Hematopoietic Tissues of vav-βgal Transgenic Mice Bearing Three Different Constructs
| Tissue . | β-gal+ Cells (%) With vav-βgal Construct* . | ||
|---|---|---|---|
| HS21/5 . | HS21/45 . | HS321/45 . | |
| Thymus | 63 ± 26 | 78 ± 13 | 87 ± 12 |
| Bone marrow | 20 ± 9 | 16 ± 6 | 19 ± 6 |
| Mac1 | 3 | 2 | 2 |
| Ter119 | 7 | 1 | 3 |
| B220 | 72 | 54 | 39 |
| IgM | 58 | 35 | 32 |
| IgD | — | 1 | 5 |
| Spleen | 17 ± 8 | 19 ± 8 | 21 ± 5 |
| B220 | 10 | 2 | 3 |
| CD4 | 36 | 37 | 21 |
| CD8 | 34 | 27 | 32 |
| Lymph node | 25 ± 13 | 34 ± 12 | 42 ± 7 |
| B220 | 5 | 0 | 1 |
| CD4 | 60 | 28 | 64 |
| CD8 | 50 | 16 | 62 |
| Tissue . | β-gal+ Cells (%) With vav-βgal Construct* . | ||
|---|---|---|---|
| HS21/5 . | HS21/45 . | HS321/45 . | |
| Thymus | 63 ± 26 | 78 ± 13 | 87 ± 12 |
| Bone marrow | 20 ± 9 | 16 ± 6 | 19 ± 6 |
| Mac1 | 3 | 2 | 2 |
| Ter119 | 7 | 1 | 3 |
| B220 | 72 | 54 | 39 |
| IgM | 58 | 35 | 32 |
| IgD | — | 1 | 5 |
| Spleen | 17 ± 8 | 19 ± 8 | 21 ± 5 |
| B220 | 10 | 2 | 3 |
| CD4 | 36 | 37 | 21 |
| CD8 | 34 | 27 | 32 |
| Lymph node | 25 ± 13 | 34 ± 12 | 42 ± 7 |
| B220 | 5 | 0 | 1 |
| CD4 | 60 | 28 | 64 |
| CD8 | 50 | 16 | 62 |
*The proportion of β-gal+ cells in a given tissue was first determined by FACS-gal analysis, and the mean and standard deviation from seven or more animals (representing at least 2 independent lines for each construct) are indicated. The background fluorescence (determined on each occasion from nontransgenic mice) was not subtracted from these values; its mean ranged from 3% for lymph node to 6% for thymus. In the representative experiments shown for populations within a tissue, the proportion of β-gal+ cells of a given type is represented as a percentage of the total cells bearing a particular marker, with the percentage for the negative control animal subtracted.