Weak Activity of vav Promoter in Transiently Transfected Cells
| Construct . | FDC-P1 . | EL-4 . | NIH3T3 . |
|---|---|---|---|
| SRα-lux | 130 | 210 | 340 |
| RSV-lux | 18 | 170 | 28 |
| HS1 vav-lux | 2.3 | 3.0 | 0.2 |
| HS21vav-lux | 3.2 | 4.6 | 0.2 |
| HS1 vavSV40-lux | 1.5 | 3.3 | 0.6 |
| HS21 vavSV40-lux | 3.5 | 1.7 | 0.8 |
| HS321 vavSV40-lux | 1.0 | 0.8 | 0.4 |
| SV40-lux | 1.0 | 1.0 | 1.0 |
| Construct . | FDC-P1 . | EL-4 . | NIH3T3 . |
|---|---|---|---|
| SRα-lux | 130 | 210 | 340 |
| RSV-lux | 18 | 170 | 28 |
| HS1 vav-lux | 2.3 | 3.0 | 0.2 |
| HS21vav-lux | 3.2 | 4.6 | 0.2 |
| HS1 vavSV40-lux | 1.5 | 3.3 | 0.6 |
| HS21 vavSV40-lux | 3.5 | 1.7 | 0.8 |
| HS321 vavSV40-lux | 1.0 | 0.8 | 0.4 |
| SV40-lux | 1.0 | 1.0 | 1.0 |
The values for luciferase activity shown, which were normalized to that of the basal SV40 promoter, derive from two experiments for EL-4 and one for FDC-P1 and NIH3T3, but comparable values were obtained in several other experiments involving all the constructs except the basal promoter.