LTC-IC Assays
| Experiment No. . | BMMC . | CD34+ (enrichment)* . | CD34− (depletion)† . |
|---|---|---|---|
| 1 | 116 ± 4 | 1880 ± 50 (16.2×) | 9.1 ± 6.5 (12.7×) |
| 2 | 52 ± 0 | 360 ± 40 (6.9×) | 3.5 ± 0 (14.8×) |
| Experiment No. . | BMMC . | CD34+ (enrichment)* . | CD34− (depletion)† . |
|---|---|---|---|
| 1 | 116 ± 4 | 1880 ± 50 (16.2×) | 9.1 ± 6.5 (12.7×) |
| 2 | 52 ± 0 | 360 ± 40 (6.9×) | 3.5 ± 0 (14.8×) |
Cells were sorted using MoAb 2E9. Fractions were grown under long-term marrow culture conditions for 5 weeks and then 5 × 104 cells were plated into duplicate CFU assays. Results are expressed as the mean ± standard error measurement of CFU-GM from duplicate plates and normalized to an input of 104cells/LTMC. Results are shown for comparative purposes. A precise numeric relationship between LTC-IC frequency and week-5 CFU-GM has not been established for dogs.
Enrichment = CFU-GM in CD34+ BMMC/CFU-GM in BMMC.
Depletion is CFU-GM in BMMC/CFU-GM in CD34− BMMC.