Binding Properties of Anti-CD34 MoAbs for CD34-Ig as Determined by BIAcore Analysis and FACS Studies
| Antibody Name . | Off Rate (L/sec) . | On Rate (L/[mol/s]) . | Kd* (nmol/L) . | 50% Saturating Concentration on 1390 (μg/mL)-151 . | 50% Saturating Concentration on ML3 (μg/mL)-151 . |
|---|---|---|---|---|---|
| 6B11 | <5 × 10−6 | 6.7 × 105 | <0.01 | 4.0 | 3.0 |
| 2E9 | <5 × 10−6 | 2.2 × 105 | <0.03 | 1.5 | 1.0 |
| 1H6 | 3.14 × 10−4 | 7.56 × 105 | 0.42 | 1.5 | 1.0 |
| 5F11-3 | 4.7 × 10−5 | 5.8 × 105 | 0.8 | 21.0 | 13.0 |
| 5F11-6 | 1.7 × 10−4 | 1.5 × 105 | 1 | 6.0 | 7.0 |
| Antibody Name . | Off Rate (L/sec) . | On Rate (L/[mol/s]) . | Kd* (nmol/L) . | 50% Saturating Concentration on 1390 (μg/mL)-151 . | 50% Saturating Concentration on ML3 (μg/mL)-151 . |
|---|---|---|---|---|---|
| 6B11 | <5 × 10−6 | 6.7 × 105 | <0.01 | 4.0 | 3.0 |
| 2E9 | <5 × 10−6 | 2.2 × 105 | <0.03 | 1.5 | 1.0 |
| 1H6 | 3.14 × 10−4 | 7.56 × 105 | 0.42 | 1.5 | 1.0 |
| 5F11-3 | 4.7 × 10−5 | 5.8 × 105 | 0.8 | 21.0 | 13.0 |
| 5F11-6 | 1.7 × 10−4 | 1.5 × 105 | 1 | 6.0 | 7.0 |
*Kd (Affinity) = off rate divided by on rate.
Determined by FACS analysis.