Table 5. Integration and Expression of... | American Society of Hematology

Table 5.

Integration and Expression of the nls-LacZ Gene in the FACS-Sorted Cell Populations

Experiment No.	Tissue	Weeks	Cytokine^5-150	Injected Cells^5-151	Genomic PCR nls-LacZ/nls-LacZ Expressionρ
					CD19⁺
						CD33⁺

14	BM	10	C2	4,500	+/+
						−/+

			C2	500	+/−
						−/+

15	BM	11	C2	6,300	+/+
						ND/+

16	BM	14	C1	8,100	−/ND
						−/ND

			C2	8,100	+/+
						+/ND

			C2	8,100	+/+
						−/ND



					CD4⁺ CD8⁺	CD4⁺ CD8⁻	CD4⁻ CD8⁺

Experiment No.	Tissue	Weeks	Cytokine^5-150	Injected Cells^5-151	Genomic PCR nls-LacZ/nls-LacZ Expressionρ
					CD19⁺
						CD33⁺

14	BM	10	C2	4,500	+/+
						−/+

			C2	500	+/−
						−/+

15	BM	11	C2	6,300	+/+
						ND/+

16	BM	14	C1	8,100	−/ND
						−/ND

			C2	8,100	+/+
						+/ND

			C2	8,100	+/+
						−/ND



					CD4⁺ CD8⁺	CD4⁺ CD8⁻	CD4⁻ CD8⁺

Thymus

6,300

+/+

−/+

6,300

+/−

+/+

−/+

Abbreviation: ND, not determined.

See footnote in Table 1.

See footnote in Table 1.

Integration of the nls-LacZ gene was determined by genomic PCR analysis.

ρ Expression of the nls-LacZ gene was determined by X-gal staining of cytospin prepared sorted cells.