Table 4. Expression of the nls-LacZ Gene... | American Society of Hematology

Table 4.

Expression of the nls-LacZ Gene in the Reconstituted BM Cells

Experiment No.	Weeks	Cytokine^4-150	Injected Cells^4-151	% Reconstitution^‡	β-Galactosidase Expression
					Cytospinρ	HPP-CFC (%)	LPP-CFC (%)	CFC-MC (%)¶
1	4	C1	8,400	54	+++	0/6	6/153 (3.9)	15/209 (7.2)
		C1	8,400	55	+++	3/24 (12.5)	17/226 (7.5)	30/246 (12.2)
		C2	6,400	26	++	0/23	5/121 (4.1)	23/260 (8.9)
		C2	6,400	29	++	3/13 (23.1)	13/130 (10)	32/263 (12.2)
		C2	6,400	47	++	1/10 (10)	3/81 (3.7)	9/177 (5)
2	4	C1	13,800	35	−	ND	ND	0/341
		C2	13,800	48	−	0/15	1/223 (0.5)	1/146 (0.7)
	7	C2	13,800	99	+	0/55	19/491 (3.9)	12/244 (4.9)
3	4	C1	7,500	43	++	11/55 (20)	66/361 (18.3)	42/215 (19.5)
		C2	5,000	29	++	4/45 (8.9)	22/215 (10.2)	24/241 (10)
		C2	5,000	50	+++	13/94 (13.8)	59/337 (17.5)	34/309 (11)
4	8	C1	5,400	66	+	0/22	4/182 (2.2)	1/153 (0.7)
		C1	5,400	26	++	1/15 (6.7)	22/217 (10.1)	12/211 (5.7)
		C1	600	42	+	1/32 (3.1)	3/151 (2.0)	4/22 (1.8)
		C1	600	75	+	0/31	0/110	0/147
		C2	5,400	77	+	0	0/39	0/20
5	8	C1	7,200	75	+	6/28 (21.4)	30/260 (11.5)	6/302 (2)
		C1	7,200	93	+	2/26 (7.7)	24/409 (5.9)	9/383 (2.4)
		C1	1,500	94	++	2/39 (5.1)	23/271 (8.5)	9/242 (3.7)
		C1	750	98	+	6/40 (15)	28/281 (10)	21/308 (6.8)
		C1	750	91	++	0/36	9/275 (3.3)	3/261 (1.2)
		C2	7,200	98	+	3/41 (7.3)	48/332 (14.5)	12/278 (4.3)
		C2	7,200	91	++	0/54	31/363 (8.5)	12/303 (4)
		C2	1,500	87	++	1/42 (2.4)	23/363 (6.4)	22/298 (7.4)
		C2	750	86	−	0/38	35/464 (7.5)	5/283 (1.8)
7	9	C2	17,600	92	−	5/112 (4.5)	17/377 (4.5)	6/181 (3.3)
		C2	2,000	74	−	0/90	3/489 (0.6)	1/156 (0.6)
		C3	17,600	51	−	0/27	2/500 (0.4)	0/199
8	10	C1	4,500	90	−	ND	ND	3/64 (4.7)
		C1	500	74	+	0/4	1/62 (1.6)	1/47 (2)
		C2	4,500	99	+	0/9	3/119 (2.5)	2/60 (3.3)
		C2	500	89	+	0/3	0/79	0/48
9	11	C2	6,300	76	−	ND	ND	6/90 (6.7)
10	14	C1	4,400	ND	ND	ND	ND	1/16 (6.3)
		C1	4,400	ND	+	1/51 (2)	2/302 (0.7)	4/245 (1.6)
		C2	4,400	ND	+	0/32	10/430 (2.3)	1/211 (2.8)
		C2	4,400	ND	+	0/18	3/290 (1)	1/73 (1.4)
11	14	C2	10,600	96	−	0/6	2/105 (1.9)	0/81
12	17	C2	4,300	99	−	ND	ND	2/114 (1.8)
		C2	4,300	97	−	0/21	0/126	0/127
		C3	13,100	95	−	0/18	1/230 (0.4)	3/225 (1.3)
		C3	4,300	95	−	ND	ND	0/78

Experiment No.	Weeks	Cytokine^4-150	Injected Cells^4-151	% Reconstitution^‡	β-Galactosidase Expression
					Cytospinρ	HPP-CFC (%)	LPP-CFC (%)	CFC-MC (%)¶
1	4	C1	8,400	54	+++	0/6	6/153 (3.9)	15/209 (7.2)
		C1	8,400	55	+++	3/24 (12.5)	17/226 (7.5)	30/246 (12.2)
		C2	6,400	26	++	0/23	5/121 (4.1)	23/260 (8.9)
		C2	6,400	29	++	3/13 (23.1)	13/130 (10)	32/263 (12.2)
		C2	6,400	47	++	1/10 (10)	3/81 (3.7)	9/177 (5)
2	4	C1	13,800	35	−	ND	ND	0/341
		C2	13,800	48	−	0/15	1/223 (0.5)	1/146 (0.7)
	7	C2	13,800	99	+	0/55	19/491 (3.9)	12/244 (4.9)
3	4	C1	7,500	43	++	11/55 (20)	66/361 (18.3)	42/215 (19.5)
		C2	5,000	29	++	4/45 (8.9)	22/215 (10.2)	24/241 (10)
		C2	5,000	50	+++	13/94 (13.8)	59/337 (17.5)	34/309 (11)
4	8	C1	5,400	66	+	0/22	4/182 (2.2)	1/153 (0.7)
		C1	5,400	26	++	1/15 (6.7)	22/217 (10.1)	12/211 (5.7)
		C1	600	42	+	1/32 (3.1)	3/151 (2.0)	4/22 (1.8)
		C1	600	75	+	0/31	0/110	0/147
		C2	5,400	77	+	0	0/39	0/20
5	8	C1	7,200	75	+	6/28 (21.4)	30/260 (11.5)	6/302 (2)
		C1	7,200	93	+	2/26 (7.7)	24/409 (5.9)	9/383 (2.4)
		C1	1,500	94	++	2/39 (5.1)	23/271 (8.5)	9/242 (3.7)
		C1	750	98	+	6/40 (15)	28/281 (10)	21/308 (6.8)
		C1	750	91	++	0/36	9/275 (3.3)	3/261 (1.2)
		C2	7,200	98	+	3/41 (7.3)	48/332 (14.5)	12/278 (4.3)
		C2	7,200	91	++	0/54	31/363 (8.5)	12/303 (4)
		C2	1,500	87	++	1/42 (2.4)	23/363 (6.4)	22/298 (7.4)
		C2	750	86	−	0/38	35/464 (7.5)	5/283 (1.8)
7	9	C2	17,600	92	−	5/112 (4.5)	17/377 (4.5)	6/181 (3.3)
		C2	2,000	74	−	0/90	3/489 (0.6)	1/156 (0.6)
		C3	17,600	51	−	0/27	2/500 (0.4)	0/199
8	10	C1	4,500	90	−	ND	ND	3/64 (4.7)
		C1	500	74	+	0/4	1/62 (1.6)	1/47 (2)
		C2	4,500	99	+	0/9	3/119 (2.5)	2/60 (3.3)
		C2	500	89	+	0/3	0/79	0/48
9	11	C2	6,300	76	−	ND	ND	6/90 (6.7)
10	14	C1	4,400	ND	ND	ND	ND	1/16 (6.3)
		C1	4,400	ND	+	1/51 (2)	2/302 (0.7)	4/245 (1.6)
		C2	4,400	ND	+	0/32	10/430 (2.3)	1/211 (2.8)
		C2	4,400	ND	+	0/18	3/290 (1)	1/73 (1.4)
11	14	C2	10,600	96	−	0/6	2/105 (1.9)	0/81
12	17	C2	4,300	99	−	ND	ND	2/114 (1.8)
		C2	4,300	97	−	0/21	0/126	0/127
		C3	13,100	95	−	0/18	1/230 (0.4)	3/225 (1.3)
		C3	4,300	95	−	ND	ND	0/78

Results of 42 BM samples subjected to the analysis are shown.

Abbreviation: ND, not determined.

F4-150

See footnote in Table 1.

F4-151

See footnote in Table 1.

‡

Percentages of reconstitution in total BM cells are shown.

ρ See footnote in Table 3.

^¶ Sum of CFU-GM, GFU-M, CFU-G, and BFU-E is presented.

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