Table 3. T-Cell Reconstitution Potential... | American Society of Hematology

Table 3.

T-Cell Reconstitution Potential of Ex Vivo–Manipulated CD34⁺⁺CD38⁻Lin⁻ Fetal Liver Cells

Experiment No.	Weeks	Cytokine^3-150	Injected Cells^3-151	Reconstitution in CD3^high Cells (%)^3-152	Expression of
					β-Galactosidaseρ
1	6	C1	8,400	ND	++
		C2	6,400	25.3	++
3	6	C1	7,500	8.5	+++
		C2	5,000	15.2	+++
9	11	C1	6,300	41.3	++
		C2	6,300	32.9	++
10	12	C1	4,400	68.0	+
		C2	4,400	33.5	+
13	16	C2	3,600	97.9	++
		C3	3,600	ND	−

Experiment No.	Weeks	Cytokine^3-150	Injected Cells^3-151	Reconstitution in CD3^high Cells (%)^3-152	Expression of
					β-Galactosidaseρ
1	6	C1	8,400	ND	++
		C2	6,400	25.3	++
3	6	C1	7,500	8.5	+++
		C2	5,000	15.2	+++
9	11	C1	6,300	41.3	++
		C2	6,300	32.9	++
10	12	C1	4,400	68.0	+
		C2	4,400	33.5	+
13	16	C2	3,600	97.9	++
		C3	3,600	ND	−

Abbreviation: ND, not determined.

F3-150

See footnote in Table 1.

F3-151

See footnote in Table 1.

F3-152

Contribution of fetal liver–derived cells was assessed based on the HLA alloantigen expression in the CD3^high population.

ρ Expression of the nls-LacZ gene was determined by X-gal staining of cytospin prepared cells. The levels of expression were scored as: −, no β-gal⁺ cells in the slide; +, 1 - 10 β-gal⁺ cells/slide; ++, 11 - 50 β-gal⁺ cells/slide; +++, >50 β-gal⁺ cells/slide.

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