In Vivo Expansion of Ex Vivo–Manipulated CD34++CD38−Lin− Fetal Liver Cells in the Human BM
| Weeks . | . | High Dose* . | Low Dose* . | ||
|---|---|---|---|---|---|
| . | . | C1† . | C2† . | C1† . | C2† . |
| 4 | Cell yield (×104) | 17.8 ± 3.2 (n = 4) | 34.6 ± 11.2 (n = 6) | ND | ND |
| (12.4-26.6) | (2.2-68.8) | ||||
| Fold expansion | 0.5 ± 0.1 (0.4-0.7) | 0.9 ± 0.3 (0.1-1.7) | ND | ND | |
| 7-11 | Cell yield (×104) | 64.5 ± 23.8 (n = 7) | 120.8 ± 23.6 (n = 9) | 54.3 ± 21.0 (n = 5) | 145.0 ± 50.3 (n = 3) |
| (5.0-181.4) | (4.1-225.3) | (12.6 ± 132.3) | (60.3-234.5) | ||
| Fold expansion | 2.1 ± 0.7 (0.5-4.5) | 5.0 ± 1.0 (0.1-8.7) | 14.1 ± 5.6 (2.5-33.1) | 57.5 ± 30.8 (15.1-117.3) | |
| Weeks . | . | High Dose* . | Low Dose* . | ||
|---|---|---|---|---|---|
| . | . | C1† . | C2† . | C1† . | C2† . |
| 4 | Cell yield (×104) | 17.8 ± 3.2 (n = 4) | 34.6 ± 11.2 (n = 6) | ND | ND |
| (12.4-26.6) | (2.2-68.8) | ||||
| Fold expansion | 0.5 ± 0.1 (0.4-0.7) | 0.9 ± 0.3 (0.1-1.7) | ND | ND | |
| 7-11 | Cell yield (×104) | 64.5 ± 23.8 (n = 7) | 120.8 ± 23.6 (n = 9) | 54.3 ± 21.0 (n = 5) | 145.0 ± 50.3 (n = 3) |
| (5.0-181.4) | (4.1-225.3) | (12.6 ± 132.3) | (60.3-234.5) | ||
| Fold expansion | 2.1 ± 0.7 (0.5-4.5) | 5.0 ± 1.0 (0.1-8.7) | 14.1 ± 5.6 (2.5-33.1) | 57.5 ± 30.8 (15.1-117.3) | |
The mean ± standard error of mean and the ranges are shown.
Abbreviation: ND, not determined.
High dose: >1 × 105 total cells (ranges: 1 - 6 × 105) that were generated from >1,500 CD34++CD38−Lin− cells; low dose: <5 × 104 cells (ranges: 1 - 5 × 104) that were generated by 500-750 CD34++CD38−Lin− cells, except for one sample received cells generated by 2,000 CD34++CD38−Lin− cells.
C1: IL-3 + IL-6 + GM-CSF + KL; C2: IL-3 + IL-6 + GM-CSF + KL + FL.