Table 1. BM Reconstitution Potential of... | American Society of Hematology

Table 1.

BM Reconstitution Potential of Ex Vivo–Manipulated CD34⁺⁺CD38⁻Lin⁻ Fetal Liver Cells

Experiment No.	Weeks	Cytokine^*	Injected Cells^†	Reconstitution (%)^‡
				Total	CD19⁺	CD33⁺	CD34⁺
1	4	C1	8,400	54	27	78	60
		C1	8,400	55	33	65	64
		C2	6,400	26	9	53	38
		C2	6,400	29	9	57	33
		C2	6,400	47	25	61	50
2	4	C2	13,800	48	36	56	53
		C1	13,800	35	10	ND	ND
	7	C2	13,800	100	89	71	73
3	4	C1	7,500	43	24	71	58
		C2	5,000	29	17	47	13
		C2	5,000	50	57	65	54
4	8	C1	5,400	66	65	66	71
		C1	5,400	26	18	56	39
		C1	600	42	22	38	23
		C1	600	75	79	50	65
		C2	5,400	77	59	83	ND
5	8	C1	7,200	75	65	82	65
		C1	7,200	93	90	93	91
		C1	1,500	94	96	90	86
		C1	1,500	10	4	37	21
		C1	750	98	98	96	91
		C1	750	91	99	98	95
		C2	7,200	98	97	99	99
		C2	7,200	91	86	97	96
		C2	1,500	87	86	87	88
		C2	750	86	73	97	98
6	8	C2	24,000	98	99	97	95
		C3	2,700	94	95	94	96
		C3	24,000	97	98	97	95
7	9	C2	17,600	92	65	92	65
		C2	2,000	74	90	93	91
		C3	17,600	51	40	27	31
8	10	C1	4,500	90	ND	ND	ND
		C1	500	74	ND	ND	ND
		C2	4,500	100	ND	ND	ND
		C2	500	89	ND	ND	ND
9	11	C2	6,300	76	ND	ND	ND
11	14	C2	10,600	96	99	92	95
12	17	C2	4,300	99	99	ND	ND
		C2	4,300	97	98	ND	ND
		C3	13,100	95	96	95	91
		C3	4,300	95	97	ND	ND

Experiment No.	Weeks	Cytokine^*	Injected Cells^†	Reconstitution (%)^‡
				Total	CD19⁺	CD33⁺	CD34⁺
1	4	C1	8,400	54	27	78	60
		C1	8,400	55	33	65	64
		C2	6,400	26	9	53	38
		C2	6,400	29	9	57	33
		C2	6,400	47	25	61	50
2	4	C2	13,800	48	36	56	53
		C1	13,800	35	10	ND	ND
	7	C2	13,800	100	89	71	73
3	4	C1	7,500	43	24	71	58
		C2	5,000	29	17	47	13
		C2	5,000	50	57	65	54
4	8	C1	5,400	66	65	66	71
		C1	5,400	26	18	56	39
		C1	600	42	22	38	23
		C1	600	75	79	50	65
		C2	5,400	77	59	83	ND
5	8	C1	7,200	75	65	82	65
		C1	7,200	93	90	93	91
		C1	1,500	94	96	90	86
		C1	1,500	10	4	37	21
		C1	750	98	98	96	91
		C1	750	91	99	98	95
		C2	7,200	98	97	99	99
		C2	7,200	91	86	97	96
		C2	1,500	87	86	87	88
		C2	750	86	73	97	98
6	8	C2	24,000	98	99	97	95
		C3	2,700	94	95	94	96
		C3	24,000	97	98	97	95
7	9	C2	17,600	92	65	92	65
		C2	2,000	74	90	93	91
		C3	17,600	51	40	27	31
8	10	C1	4,500	90	ND	ND	ND
		C1	500	74	ND	ND	ND
		C2	4,500	100	ND	ND	ND
		C2	500	89	ND	ND	ND
9	11	C2	6,300	76	ND	ND	ND
11	14	C2	10,600	96	99	92	95
12	17	C2	4,300	99	99	ND	ND
		C2	4,300	97	98	ND	ND
		C3	13,100	95	96	95	91
		C3	4,300	95	97	ND	ND

Results of 42 BM samples that were reconstituted by injected fetal liver cells are shown.

Abbreviation: ND, not determined.

C1: IL-3 + IL-6 + GM-CSF + KL; C2: IL-3 + IL-6 + GM-CSF + KL + FL; C3: IL-6 + KL + FL.

Number of injected cells are indicated as the number of CD34⁺⁺CD38⁻Lin⁻ cells at the initiation of culture.

Contribution of fetal liver–derived cells was assessed based on the HLA alloantigen expression.