TRAPase-Positive Cell and Multinucleated Cell Formation From Fractionated Cells in the Presence of CESJ Medium, M-CSF, and GM-CSF
| . | % TRAPase-Positive Cells . | Multinucleated TRAPasePositive Cells/Well . | n . |
|---|---|---|---|
| O-CSF | 98.6 ± 3.1 | 5.8 ± 4.3 | 8 |
| M-CSF | 0 | 0 | 8 |
| GM-CSF | 3.2 ± 4.1 | 0 | 8 |
| . | % TRAPase-Positive Cells . | Multinucleated TRAPasePositive Cells/Well . | n . |
|---|---|---|---|
| O-CSF | 98.6 ± 3.1 | 5.8 ± 4.3 | 8 |
| M-CSF | 0 | 0 | 8 |
| GM-CSF | 3.2 ± 4.1 | 0 | 8 |
c-kithigh or c-kitlow cells (500 cells/well) were cultured in 4-well chamber slides in the presence of CESJ medium, M-CSF, or GM-CSF for 14 days. The cells in four random fields per chamber were enumerated as TRAPase-positive or -negative type. Whole fields were examined to identify TRAPase-positive multinucleated cells (>3 nuclei). Because the results for c-kithigh and c-kitlow cells were the same, n reflects the combined total wells.