Effect of BCR/ABL Protein Expression on Colony Formation of 32Dcl3 and BMC
| Cells . | IL-3 . | Vector . | Wild-Type . | K1172R . | ΔSH3 . |
|---|---|---|---|---|---|
| 32Dcl3 | − | 0 | 994 ± 340 | 0 | 860 ± 192 |
| BMC | − | 0 | 7 ± 2 | 0 | 6 ± 3 |
| BMC | + | 31 ± 8 | 95 ± 17 | 35 ± 12 | 83 ± 13 |
| Cells . | IL-3 . | Vector . | Wild-Type . | K1172R . | ΔSH3 . |
|---|---|---|---|---|---|
| 32Dcl3 | − | 0 | 994 ± 340 | 0 | 860 ± 192 |
| BMC | − | 0 | 7 ± 2 | 0 | 6 ± 3 |
| BMC | + | 31 ± 8 | 95 ± 17 | 35 ± 12 | 83 ± 13 |
Colonies from 104 32Dcl3 cells (individual clones expressing the indicated proteins) or 105 BMC (infected with the viruses carrying the indicated constructs) were counted 12 days after plating in MethoCult H4230 semisolid medium with or without 0.1 U/mL rmuIL-3. Results are the mean ± SD from three independent experiments.