Effects of bcr-abl Ribozymes in Cell Lines
| Reference . | Cell Line . | Transfection Method . | Robozyme . | Effects . |
|---|---|---|---|---|
| 74,91 | K562 | DOTAP | Unmodified RNA | Uptake followed by fluorescence, ribozyme stable up to 12 hours Decrease in proliferation |
| 75 | EM-2, HL60 | Transfectam | DNA-RNA hybrid | Decrease inbcr-abl mRNA, protein expression, growth |
| (lipopolyamine) | No observable effects on p160bcr levels, β-actin mRNA unaffected Unrelated ribozyme had very limited effect | |||
| 93 | K562 | Various vectors | Decrease inbcr-abl kinase activity, cell number | |
| Vector and ribozyme expression level dependent (RNA polymerase III tRNA vector best) | ||||
| 76 | 32Dbcr-abl | Lipofectin Folic acid-poly lysine | Unmodified RNA | Slight effect on mRNA levels Decrease in bcr-abl mRNA levels Inactive ribozyme had no effect on mRNA levels |
| 77 | 32Db3a2 in SCID mice | Lipofectamine | DNA-RNA hybrid | Increase in survival of SCID mice after injection with ribozyme treated 32Db3a2 cells |
| Reference . | Cell Line . | Transfection Method . | Robozyme . | Effects . |
|---|---|---|---|---|
| 74,91 | K562 | DOTAP | Unmodified RNA | Uptake followed by fluorescence, ribozyme stable up to 12 hours Decrease in proliferation |
| 75 | EM-2, HL60 | Transfectam | DNA-RNA hybrid | Decrease inbcr-abl mRNA, protein expression, growth |
| (lipopolyamine) | No observable effects on p160bcr levels, β-actin mRNA unaffected Unrelated ribozyme had very limited effect | |||
| 93 | K562 | Various vectors | Decrease inbcr-abl kinase activity, cell number | |
| Vector and ribozyme expression level dependent (RNA polymerase III tRNA vector best) | ||||
| 76 | 32Dbcr-abl | Lipofectin Folic acid-poly lysine | Unmodified RNA | Slight effect on mRNA levels Decrease in bcr-abl mRNA levels Inactive ribozyme had no effect on mRNA levels |
| 77 | 32Db3a2 in SCID mice | Lipofectamine | DNA-RNA hybrid | Increase in survival of SCID mice after injection with ribozyme treated 32Db3a2 cells |